Product Details

Purity

>85% (SDS-PAGE)

Target Names

amtB

Uniprot No.

P69681

Alternative Names

amtB; ybaG; b0451; JW0441Ammonia channel; Ammonia transporter

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)). Transport is electrogenic. Following sequestration of NH(4)(+) at the periplasmic face, NH(4)(+) is deprotonated and neutral NH(3) is transported into the cytoplasm. Neutral NH(3) and charged H(+) are carried separately across the membrane on a unique two-lane pathway, before recombining to NH(4)(+) inside the cell. In vitro can also transport the larger analogs methylamine and methylammonium. Also acts as a sensor of the extracellular ammonium concentration.

Gene References into Functions

Amt and Rh proteins are not functionally equivalent and that permeation takes place according to two distinct mechanisms. PMID: 24021113
the twin-histidine element serves as a filter to prevent K(+) conduction in Escherichia coli ammonium channel AmtB PMID: 23546877
Data explore the similarity and difference of the conduction mechanism of methylamine and ammonia molecules through AmtB, using molecular dynamics simulations. PMID: 20973592
structure of an ammonia channel AmtB, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times; reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3 PMID: 15361618
solved the structure of wild-type AmtB from Escherichia coli in two crystal forms at 1.8- and 2.1-A resolution; binding site and hydrophobic pore are described; adiabatic free energy calculations support an electrostatic barrier model for specificity PMID: 15563598
Review. The Escherichia coli ammonium transport protein (AmtB) has become the model system of choice for analysis of the process of ammonium uptake by the ubiquitous Amt family of inner membrane proteins. PMID: 16563828
Co-purification of AmtB with GlnK from ammonium-shocked E. coli cells definitvely demonstrates that these two proteins form a functional complex with a stoichiometry of 1:1. PMID: 16864585
simulations showed that the entrance of NH(4)(+) into the periplasmic recruitment vestibule requires only 3.1 kcal/mol of energy PMID: 16910683
in Escherichia coli AmtB, both histidines are absolutely required for optimum substrate conductance PMID: 17040913
GlnK interacts with AmtB almost exclusively via a long surface loop containing Y51 (T-loop), the tip of which inserts deeply into the cytoplasmic pore exit, blocking ammonia conduction PMID: 17220269
the C-terminus play a significant role in normal AmtB function and the C-terminal region might also mediate co-operativity between the three subunits of AmtB. PMID: 17453422
The GlnK protein binding causes a significant downshift in the Am6 pK(a) value of the AmtB. However, this downshift is perfectly compensated by the reorientation of the protein backbone upon AmtB-GlnK complex formation. PMID: 17707821
AmtB allows accumulation of CH(3)NH(3)(+) ion in response to the electrical potential across the membrane PMID: 17998534
AmtB activity absolutely requires F215 but not F107 PMID: 18362341

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Ammonia transporter channel (TC 1.A.11.2) family

Database Links

KEGG: ecj:JW0441

STRING: 316385.ECDH10B_0407

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Recombinant Escherichia coli Ammonia channel (amtB), partial

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