Recombinant Escherichia coli Crossover junction endodeoxyribonuclease RuvC (ruvC)

The recombinant Escherichia coli (strain K12) ruvC was expressed with the amino acid range of 2-173. The theoretical molecular weight of the ruvC protein is 34.6 kDa. Expression of this ruvC protein is conducted in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the ruvC encoding gene fragment was conducted, allowing for easier detection and purification of the ruvC protein in subsequent stages.

The Escherichia coli crossover junction endodeoxyribonuclease RuvC is a key enzyme involved in the resolution of Holliday junctions during genetic recombination and DNA repair. As part of the RuvABC complex, RuvC works in coordination with RuvA and RuvB to process Holliday junctions formed during DNA recombination events. RuvC acts specifically at the junction point, symmetrically cleaving the DNA strands, leading to the separation and resolution of the crossover structure. This process is crucial for the accurate segregation of genetic material during cell division and the repair of DNA damage. RuvC's activity is tightly regulated and orchestrated within the RuvABC complex, highlighting its importance in maintaining genomic integrity in Escherichia coli. Understanding the molecular mechanisms of RuvC provides insights into the intricate processes of DNA repair and recombination, contributing to the broader knowledge of genome stability and maintenance.