Product Details

Purity

>85% (SDS-PAGE)

Target Names

rpoB

Uniprot No.

P0A8V2

Alternative Names

rpoB; groN; nitB; rif; ron; stl; stv; tabD; b3987; JW3950; DNA-directed RNA polymerase subunit beta; RNAP subunit beta; EC 2.7.7.6; RNA polymerase subunit beta; Transcriptase subunit beta

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.; Resistance to the antibiotics salinamide A, salinamide B, rifampicin, streptolydigin, CBR703, myxopyronin, and lipiarmycin can result from mutations in this protein.; Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNAP. It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis.

Gene References into Functions

The C-terminal segment of coliphage HK022 Nun interacts with the RNA polymerase beta and beta' subunits inside the RNA polymerase active site cleft as well as with nearly every element of the nucleic acid scaffold, essentially crosslinking the RNA polymerase and the nucleic acids to prevent translocation. PMID: 28318486
The properties of generated RNA polymerase (RNAP) beta subunit variants Iindicate an RNA/protein interaction network that is crucial for transcription, thereby explaining the details of initiation-to-elongation transition on atomic level. PMID: 29036608
The D654Y single amino acid substitution in RpoB rendered E. coli cells resistant to osmotic stress, probably due to improved cell growth and viability via enhanced sugar uptake under stressed conditions, and activated a potential "pre-defense" mechanism under non-stressed conditions. The findings of this work will be useful for bacterial host improvement to enhance its resistance to osmotic stress and facilitate bio-base PMID: 28193207
Genotype-by-environment interactions of rpoB generated by antibiotic resistance mutations are more pronounced after adaptation to other types of environmental variation, making it difficult to predict long-term selection PMID: 23701170
analysis of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli PMID: 23223236
data suggest that contact of the RNA polymerase beta subunit flap-tip helix hydrophobic patch to the sigma region 4 hydrophobic core is essential for stable interaction of the flap-tip helix with region 4 PMID: 15465046
Amino acid substitutions define a highly conserved surface-exposed patch on the beta1 domain of RNA polymerase beta subunit that is required for the initial step of transcription-coupled repair. PMID: 15687384
dG1551 and dC1692 in rpoB are critical for rifampicin drug resistance. PMID: 16276894
Data report the structure of Escherichia coli GreB, and show that the carboxy-terminal coiled-coil of the RNA polymerase beta'-subunit is the main binding site for Gre factors. PMID: 17917675
RNA polymerase is able to bind and react with many different RNAs and we suggest that RNAs are involved in transcriptional regulation more frequently than anticipated. PMID: 18388495

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Protein Families

RNA polymerase beta chain family

Database Links

KEGG: ecj:JW3950

STRING: 316385.ECDH10B_4175

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Recombinant Escherichia coli DNA-directed RNA polymerase subunit beta (rpoB), partial

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