Product Details

Purity

>85% (SDS-PAGE)

Target Names

flhD

Uniprot No.

P0A8S9

Alternative Names

flhD; flbB; b1892; JW1881Flagellar transcriptional regulator FlhD

Species

Escherichia coli (strain K12)

Expression Region

1-116

Target Protein Sequence

MHTSELLKHI YDINLSYLLL AQRLIVQDKA SAMFRLGINE EMATTLAALT LPQMVKLAET NQLVCHFRFD SHQTITQLTQ DSRVDDLQQI HTGIMLSTRL LNDVNQPEEA LRKKRA

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.

Gene References into Functions

three IS elements, IS1, IS3 and IS5, transpose to multiple upstream sites within a 370 bp region of the flhDC operon control region. The data are consistent with the possibility that appropriate environmental conditions, namely those that permit but hinder flagellar rotation, result in the activation of a mutational pathway that involves IS element insertion upstream of the flhDC operon. PMID: 28100305
Mutations in the flhD can exert positive or negative effects on motility. PMID: 27821046
FlhD is part of flagellar regulatory network. PMID: 25275371
Upregulation of fliZ by NtrC-sigma(N) was shown to be indirect and required an intact flagellar regulator flhDC. PMID: 24931910
Authors report that not only integrations of IS insertion sequences into the regulatory region of the flhDC operon, but also a missense mutation in the lrhA gene enhances motility by relieving transcriptional repression of the flhDC operon. PMID: 23456724
CsrA activates flhDC expression by protecting flhDC mRNA from RNase E-mediated cleavage. PMID: 23305111
Study demonstrates that insertion sequence IS5 in Escherichia coli inserts into the upstream region of the flhDC operon in a manner that depends on whether the environment permits motility. PMID: 21390082
Mutations in the flhD do not affect cell division. PMID: 20546312
These results indicate that the accumulation of sigmaS is one of the causes of the repression of transcription of flhD. PMID: 20185506
IS element insertion may activate transcription of the flhD operon by reducing transcriptional repression. PMID: 15516564
The data indicate a wider FlhD(2)C(2) regulon, in which non-flagellar genes are bound and activated directly PMID: 15941987
flhDC mutants of Escherichia coli are nonmotile, better colonize in the intestine, and better utilize sugars than wild type bacteria and Entner-Doudoroff pathway mutants. PMID: 16299298
crystallographic structure of the Escherichia coli FlhDC complex has been solved to 3.0 A resolution PMID: 16337229

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Subcellular Location

Cytoplasm.

Protein Families

FlhD family

Database Links

KEGG: ecj:JW1881

STRING: 316385.ECDH10B_2033

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Code	CSB-MP359186ENV
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Recombinant Escherichia coli Flagellar transcriptional regulator FlhD (flhD)

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