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Recombinant Escherichia coli HTH-type transcriptional regulator iscR (iscR)

Recombinant Escherichia coli HTH-type transcriptional regulator iscR (iscR)

Code	CSB-YP314598ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP314598ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP314598ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP314598ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP314598ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

iscR

Uniprot No.

P0AGK8

Alternative Names

iscR; yfhP; b2531; JW2515; HTH-type transcriptional regulator IscR

Species

Escherichia coli (strain K12)

Expression Region

1-162

Target Protein Sequence

MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR KNGLVSSVRG PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG GDKCLTHALW RDLSDRLTGF LNNITLGELV NNQEVLDVSG RQHTHDAPRT RTQDAIDVKL RA

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.

Gene References into Functions

findings show how an sRNA, RyhB, and a transcription factor, IscR, regulate expression of an essential gene, erpA, in E. coli PMID: 27651365
Authors demonstrate that in the absence of isc, IscR-dependent suf activation is essential since strains lacking both the Isc pathway and IscR were not viable unless Suf was expressed ectopically. PMID: 25266384
These data suggest a unique mechanism of ligand-mediated DNA site recognition, whereby metallocluster ligation relocates a protein-specificity determinant to expand DNA target-site selection, allowing a broader transcriptomic response by holo-IscR. PMID: 23644595
Fe-S clusters directed at the apo-NsrR and apo-IscR proteins are built on either of the two scaffolds, IscU or SufB. PMID: 23320508
Together, these data show how transcriptional control of iscRSUA-hscBA-fdx by [2Fe-2S]-IscR allows E. coli to respond efficiently to varying Fe-S demands. PMID: 23075318
a combination of ArcA and AppY antirepression of IscR function, lower levels of IscR, and weak repression by [2Fe-2S]-IscR leads to increased hyaA promoter expression under anaerobic conditions PMID: 23065979
These results suggest that, IscR stabilizes LexA at the cka promoter after DNA damage thus, preventing its cleavage and inactivation, and this cooperation ensures that suicidal colicin K production is switched on only as a last resort. PMID: 22812562
The findings presented here indicate a more general role of IscR in the regulation of Fe-S cluster biogenesis and that IscR contributes to the O(2) regulation of several promoters controlling the expression of anaerobic Fe-S proteins. PMID: 16677314
The iscR null mutation abolished oxidant-responsive elements binding activity in cell extracts. PMID: 16824106
Activation of the suf operon by IscR occurs only under conditions where Fur loses its repressor activity. PMID: 18849427