Recombinant Escherichia coli Manganese transport protein MntH (mntH), partial

Code CSB-YP358929ENV
MSDS
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Source Yeast
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Code CSB-EP358929ENV
MSDS
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Source E.coli
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Code CSB-EP358929ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP358929ENV
MSDS
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Source Baculovirus
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Code CSB-MP358929ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mntH
Uniprot No.
Alternative Names
mntH; yfeP; b2392; JW2388; Divalent metal cation transporter MntH
Species
Escherichia coli (strain K12)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
H(+)-stimulated, divalent metal cation uptake system. Involved in manganese and iron uptake. Can also transport cadmium, cobalt, zinc and to a lesser extent nickel and copper. Involved in response to reactive oxygen.
Gene References into Functions
  1. Synthetic peptides corresponding to the sixth transmembrane segment of MntH(Proton-dependent Manganese Transporter) and its two mutations in the functionally important conserved histidine residue were used as a model for structure-function study of MntH. PMID: 20517953
  2. Multiple conserved residues on transmembrane segment 6 of MntH may comprise an important face that is critical for protein PMID: 20441230
  3. Study reveals structural and functional roles for four Nramp-specific transmembrane residues in MntH that may have contributed to the emergence of this family of metal and proton cotransporters. PMID: 15641799
  4. Failure to induce MntH results in a profound growth defect during H2O2 stress, and mutants that cannot import manganese are unable to sequester iron. PMID: 19400769
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
NRAMP family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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