Product Details

Purity

>85% (SDS-PAGE)

Target Names

mdfA

Uniprot No.

P0AEY8

Alternative Names

mdfA; cmlA; cmr; b0842; JW0826; Multidrug transporter MdfA; Chloramphenicol resistance pump Cmr

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. Overexpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na(+) or K(+).

Gene References into Functions

MdfA has exceptional promiscuous capabilities not only in its interaction with a plethora of chemically dissimilar compounds, but also concerning the various faces of its modes of energy consumption. [review] PMID: 28951231
Crystallization and X-ray diffraction analysis of the multidrug-resistance transporter MdfA from Escherichia coli has been reported. PMID: 28695852
The MdfA structure consists of four pseudo-symmetrical, three-helix repeats, consistent with the typical structure of an MFS transporter. PMID: 26238402
The mdfA gene can function as transporter responsible for multidrug resistance, and also mediated the synergistic function with its surrounding genes in conjugative plasmids. PMID: 24684286
GlpG is able to recognize and cleave truncated forms of MdfA but not the intact protein. PMID: 19919105
results show that MdfA confers extreme alkaline pH resistance and allows the growth of transformed cells under conditions that are close to those used normally by alkaliphiles (up to pH 10) by maintaining a physiological internal pH PMID: 15371593
results suggest that in the absence of specific chloramphenicol selection pressure, the cmlA gene is maintained by virtue of gene linkage to genes encoding resistance to antimicrobials that are currently approved for use in food animals PMID: 15668031
Arg112 is important for multidrug transport by MdfA. PMID: 16274234
properties of MdfA including its multidrug recognition capacity, proton recognition determinants, aspects of energy utilization, and physiological role [review] PMID: 17114895
the Mdr-binding pocket of MdfA is conformationally sensitive PMID: 19808670
In this study, we got an evidence that MdfA is a chloramphenicol/H+ antiporter. PMID: 9644262

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Major facilitator superfamily, MdfA family

Database Links

KEGG: ag:CAA69997

STRING: 316385.ECDH10B_0911

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Recombinant Escherichia coli Multidrug transporter MdfA (mdfA), partial

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