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Recombinant Escherichia coli Multiple antibiotic resistance protein marA (marA)

Recombinant Escherichia coli Multiple antibiotic resistance protein marA (marA)

Code	CSB-YP359773ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP359773ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP359773ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP359773ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP359773ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

marA

Uniprot No.

P0ACH5

Alternative Names

marA; b1531; JW5249Multiple antibiotic resistance protein MarA

Species

Escherichia coli (strain K12)

Expression Region

1-127

Target Protein Sequence

MSRRNTDAIT IHSILDWIED NLESPLSLEK VSERSGYSKW HLQRMFKKET GHSLGQYIRS RKMTEIAQKL KESNEPILYL AERYGFESQQ TLTRTFKNYF DVPPHKYRMT NMQGESRFLH PLNHYNS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

May be a transcriptional activator of genes involved in the multiple antibiotic resistance (Mar) phenotype. It can also activate genes such as sodA, zwf and micF.

Gene References into Functions

Transcription factors MarR and MarA confer multidrug resistance in enteric bacteria by modulating efflux pump and porin expression. Here, the authors show that MarA also upregulates genes required for lipid trafficking and DNA repair, thus reducing antibiotic entry and quinolone-induced DNA damage. PMID: 29133912
Replacement of the MarA glutamic acid residue 89 with alanine greatly increased the marbox binding and activation of many class I promoters. PMID: 21097628
roles of the Escherichia coli transcriptional regulators MarA, SoxS and Rob in susceptibility to cationic antimicrobial peptides PMID: 19926649
A model of the ternary complex shows that alpha-CTD (carboxy-terminal domain of the alpha subunit of RNA polymerase) uses a '265-like determinant' to contact MarA at a surface distant from the DNA. PMID: 15458404
Data show that expression of TetD activates transcription of a subset of the SoxS/MarA/Rob regulon genes and confers resistance to redox-cycling compounds and antibiotics. PMID: 15853893
analysis of marA-mediated transcriptional repression of the rob promoter PMID: 16478729
Findings show that repression of hdeAB by MarA depends on pH, growth phase, and other regulators of hdeAB and is associated with reduced resistance to acid conditions. PMID: 18083817
Results describe the activation of the Escherichia coli marA/soxS/rob regulon in response to transcriptional activator concentration. PMID: 18514222
The authors show here that tolC has two additional strong overlapping promoters, p3 and p4, which are downstream of p1, p2 and the marbox and are activated by MarA, SoxS and Rob. PMID: 18673442