Recombinant Escherichia coli Oxygen-insensitive NADPH nitroreductase(nfsA)

Code CSB-YP323751ENV
Size US$2959
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names nfsA
Uniprot No. P17117
Research Area Others
Alternative Names nfsA; mda18; mdaA; ybjB; b0851; JW0835; Oxygen-insensitive NADPH nitroreductase; EC 1.-.-.-; Modulator of drug activity A
Species Escherichia coli (strain K12)
Source Yeast
Expression Region 1-240aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 28.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.
Gene References into Functions
  1. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin. PMID: 29753133
  2. reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism PMID: 27986535
  3. Findings revealed that Phe42 is important for maintaining NfsA activity and structure. PMID: 23801116
  4. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate. PMID: 22173483
Protein Families Flavin oxidoreductase frp family
Database Links

KEGG: ecj:JW0835

STRING: 316385.ECDH10B_0921


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