Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

nfsA

Uniprot No.

P17117

Research Area

Others

Alternative Names

nfsA; mda18; mdaA; ybjB; b0851; JW0835; Oxygen-insensitive NADPH nitroreductase; EC 1.-.-.-; Modulator of drug activity A

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-240aa

Target Protein Sequence

MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

42.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-240 constitute the expression domain of recombinant Escherichia coli (strain K12) nfsA. The calculated molecular weight for this nfsA protein is 42.8 kDa. This nfsA protein is produced using e.coli expression system. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of nfsA, which enables a simple process of detecting and purifying the nfsA recombinant protein in the following steps.

Escherichia coli oxygen-insensitive NADPH nitroreductase, encoded by the nfsA gene, is an enzyme that belongs to the nitroreductase family. This enzyme is involved in the reduction of nitroaromatic compounds using NADPH as a cofactor. The NfsA enzyme plays a role in the detoxification of nitroaromatic compounds by catalyzing the reduction of nitro groups to amino groups. This process is essential for bacterial resistance to nitro-containing antibiotics and for the metabolism of certain environmental pollutants. Understanding the structure and function of NfsA contributes to the knowledge of bacterial adaptation to diverse environments.

Target Background

Function

Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.

Gene References into Functions

Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin. PMID: 29753133
reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism PMID: 27986535
Findings revealed that Phe42 is important for maintaining NfsA activity and structure. PMID: 23801116
NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate. PMID: 22173483

Protein Families

Flavin oxidoreductase frp family

Database Links

KEGG: ecj:JW0835

STRING: 316385.ECDH10B_0921

Code	CSB-EP323751ENV
Abbreviation	Recombinant E.coli nfsA protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP323751ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) nfsA. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP323751ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) nfsA.
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Recombinant Escherichia coli Oxygen-insensitive NADPH nitroreductase (nfsA)

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