Recombinant Escherichia coli Oxygen-insensitive NADPH nitroreductase (nfsA)

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Code CSB-EP323751ENV
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP323751ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) nfsA.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP323751ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) nfsA.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
nfsA
Uniprot No.
Research Area
Others
Alternative Names
nfsA; mda18; mdaA; ybjB; b0851; JW0835; Oxygen-insensitive NADPH nitroreductase; EC 1.-.-.-; Modulator of drug activity A
Species
Escherichia coli (strain K12)
Source
E.coli
Expression Region
1-240aa
Target Protein Sequence
MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
42.8kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Amino acids 1-240 constitute the expression domain of recombinant Escherichia coli (strain K12) nfsA. The calculated molecular weight for this nfsA protein is 42.8 kDa. This nfsA protein is produced using e.coli expression system. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of nfsA, which enables a simple process of detecting and purifying the nfsA recombinant protein in the following steps.

Escherichia coli oxygen-insensitive NADPH nitroreductase, encoded by the nfsA gene, is an enzyme that belongs to the nitroreductase family. This enzyme is involved in the reduction of nitroaromatic compounds using NADPH as a cofactor. The NfsA enzyme plays a role in the detoxification of nitroaromatic compounds by catalyzing the reduction of nitro groups to amino groups. This process is essential for bacterial resistance to nitro-containing antibiotics and for the metabolism of certain environmental pollutants. Understanding the structure and function of NfsA contributes to the knowledge of bacterial adaptation to diverse environments.

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Target Background

Function
Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.
Gene References into Functions
  1. Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin. PMID: 29753133
  2. reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism PMID: 27986535
  3. Findings revealed that Phe42 is important for maintaining NfsA activity and structure. PMID: 23801116
  4. NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate. PMID: 22173483
Protein Families
Flavin oxidoreductase frp family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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