Name: nfsA Antibody
Brand: CUSABIO
SKU: CSB-PA323751XA01ENV

Product Details

Full Product Name	Rabbit anti-Escherichia coli (strain K12) nfsA Polyclonal antibody
Uniprot No.	P17117
Target Names	nfsA
Alternative Names	nfsA antibody; mda18 antibody; mdaA antibody; ybjB antibody; b0851 antibody; JW0835 antibody; Oxygen-insensitive NADPH nitroreductase antibody; EC 1.-.-.- antibody; Modulator of drug activity A antibody
Raised in	Rabbit
Species Reactivity	Escherichia coli (strain K12)
Immunogen	Recombinant Escherichia coli (strain K12) nfsA protein
Immunogen Species	Escherichia coli (strain K12)
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Protein A/G
Concentration	It differs from different batches. Please contact us to confirm it.
Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Tested Applications	ELISA, WB (ensure identification of antigen)
Protocols	ELISA Protocol
Troubleshooting and FAQs	Antibody FAQs
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables	① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control);
Quality Guarantee	① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive;
Lead Time	Made-to-order (14-16 weeks)

Target Background

Function (From Uniprot)	Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.
Gene References into Functions	Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin. PMID: 29753133 reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism PMID: 27986535 Findings revealed that Phe42 is important for maintaining NfsA activity and structure. PMID: 23801116 NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate. PMID: 22173483
Protein Families	Flavin oxidoreductase frp family
Database Links	KEGG: ecj:JW0835 STRING: 316385.ECDH10B_0921

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nfsA Antibody

Code	CSB-PA323751XA01ENV
Size	Enquire
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