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Recombinant Escherichia coli Probable phospholipid-binding lipoprotein mlaA (mlaA)

Recombinant Escherichia coli Probable phospholipid-binding lipoprotein mlaA (mlaA)

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Code	CSB-EP300563ENV
Abbreviation	Recombinant E.coli mlaA protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

mlaA

Uniprot No.

P76506

Research Area

Others

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

18-251aa

Target Protein Sequence

CASSGTDQQGRSDPLEGFNRTMYNFNFNVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMADGFYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQAIQDDLKDIDSE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant Escherichia coli mlaA protein is produced by cloning the gene encoding the full-length mature Escherichia coli probable phospholipid-binding lipoprotein mlaA (18-251aa) and the N-terminal 6xHis-tag gene into a plasmid and transferring the recombinant plasmid into E.coli cells for protein production. The recombinant mlaA protein is isolated and purified from the cell lysate. Its purity is greater than 90% measured by SDS-PAGE. On the gel, the recombinant mlaA protein appears as a band with a molecular weight of approximately 31 kDa.

The probable phospholipid-binding lipoprotein mlaA is essential for maintaining the integrity and functionality of the outer membrane in E. coli by regulating the transport and recycling of phospholipids.

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Target Background

Function

Involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.

Gene References into Functions

established that OmpC is an additional component of the Mla system; the OmpC-MlaA complex may function to remove phospholipids directly from the outer leaflet to maintain outer membrane lipid asymmetry;work reveals a novel function for the general diffusion channel OmpC in lipid transport PMID: 26314242

Subcellular Location

Cell outer membrane; Lipid-anchor.

Protein Families

MlaA family

Database Links

KEGG: ecj:JW2343

STRING: 316385.ECDH10B_2509

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