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| Code | CSB-PA300563XA01ENV |
| Size | US$299 |
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The mlaA polyclonal antibody production involves the repetitive immunization of a rabbit with recombinant Escherichia coli (strain K12) mlaA protein (18-251aa). Following the attainment of an adequate antibody titer, the rabbit is bled, and antibodies are purified from the serum utilizing affinity chromatography. The efficacy of the resulting mlaA antibody is then assessed in ELISA and WB applications, revealing its reactivity with Escherichia coli (strain K12) mlaA protein.
The mlaA protein in Escherichia coli (strain K12) serves a pivotal role in the Mla (maintenance of lipid asymmetry) pathway, actively participating in the preservation of the asymmetry of the outer membrane. Its primary function involves preventing the accumulation of phospholipids in the outer leaflet of the outer membrane by facilitating their retrograde transport to the inner membrane. By preventing phospholipid mislocalization, mlaA ensures the selective permeability and barrier function of the outer membrane, ultimately supporting the structural stability and functionality of Escherichia coli in diverse environmental conditions.
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KEGG: ecj:JW2343
STRING: 316385.ECDH10B_2509
