Recombinant Escherichia coli Prophage outer membrane lipoprotein RzoR (rzoR)

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Code CSB-EP349921ENV
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
rzoR
Uniprot No.
Research Area
Others
Alternative Names
rzoR; b4528; JW5213; Prophage outer membrane lipoprotein RzoR; o-spanin; Outer membrane lipoprotein Rz1 from lambdoid prophage Rac; Spanin from lambdoid prophage Rac; outer membrane subunit
Species
Escherichia coli (strain K12)
Source
E.coli
Expression Region
20-61aa
Target Protein Sequence
CTSKQSVSQCVKPPPPPAWIMQPPPDWQTPLNGIISPSGNDW
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
19.9 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-KSI-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

In the general approach to express the recombinant Escherichia coli (strain K12) rzoR protein, a plasmid encoding the Escherichia coli (strain K12) rzoR protein (20-61aa) is first constructed. The constructed plasmid is then introduced into e.coli cells. Plasmid-containing e.coli cells are screened and cultured under conditions that induce the protein expression. The protein is fused with a N-terminal 6xHis-KSI tag. Lysing the cultured cells and purifying the resulting recombinant rzoR protein through affinity purification. The SDS-PAGE analysis is conducted to confirm the presence of the recombinant rzoR protein and assess its purity. Its purity is over 85%.

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Target Background

Function
Component of the spanin complex that disrupts the outer membrane and causes cell lysis during virus exit. The spanin complex conducts the final step in cell lysis by disrupting the outer membrane after holin and endolysin action have permeabilized the inner membrane and degraded the host peptidoglycans.
Subcellular Location
Cell outer membrane; Lipid-anchor; Periplasmic side.
Protein Families
Lambdalikevirus o-spanin family
Database Links
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