Recombinant Escherichia coli Putative kinase YjjJ (yjjJ)

Code CSB-YP334558ENV
MSDS
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Source Yeast
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Code CSB-EP334558ENV
MSDS
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Source E.coli
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Code CSB-EP334558ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334558ENV
MSDS
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Source Baculovirus
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Code CSB-MP334558ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
yjjJ
Uniprot No.
Alternative Names
yjjJ; b4385; JW4348Toxin YjjJ; Putative DNA-binding transcriptional regulator YjjJ; Putative serine/threonine kinase toxin YjjJ; EC 2.-.-.-
Species
Escherichia coli (strain K12)
Expression Region
1-443
Target Protein Sequence
MSELTDLLLQ GPRSAPELRQ RLAISQATFS RLVAREDRVI RFGKARATRY ALLRPYRGIE RIPVWRVDDT GKAHKFADIR LCWPQGSCLV TGADGDEQWF DGLPWYLTDL RPQGFLGRAW GRKLAAQLNL TDDIRLWQEE DVLYALTVFN GEYTGGWLVG EGNYQRWITA QHPAEIPLDQ KLTHYEQLAS DALAGEIVGS SAGGEQPKFT YYAQTPSGNK HVLVKFTVPQ QTAVSQRWGD LLIAESIAAQ ILRDGGIHAI ESTVLVTSNR QVFLEAERFD CKGNDGRLPI VSLEAVQSEF ISSPGSWPQA MRRLCEQQLV THQSVAQTEV IWAFGRLIAN SDMHAGNLSF YLSEPPFALT PVYDMLPMVY APNSAGMLRD AAIEVKFDLN VSKSAWLTAI PLAQQFWQTV ARDPRISEAF RHIAQEMPEK IRQIEEKVAR MGG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Toxic when overexpressed in E.coli, leading to long filamentous cells. The toxic effect is neutralized by non-cognate antitoxin HipB. Does not seem to inhibit DNA, RNA or protein synthesis, and unlike paralogous toxin HipA its toxic activity is not counteracted by overexpression of GltX. Binds DNA. Might be a protein kinase.
Gene References into Functions
  1. Despite being a homolog of HipA, YjjJ seems to have different cellular target(s) than that of HipA. HipB, the cognate antitoxin of HipA, also acts as an antitoxin for YjjJ. PMID: 28430938
Protein Families
HipA Ser/Thr kinase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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