Recombinant Escherichia coli RNA pyrophosphohydrolase (rppH)

Code CSB-YP363964ENV
MSDS
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Source Yeast
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Code CSB-EP363964ENV
MSDS
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Source E.coli
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Code CSB-EP363964ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP363964ENV
MSDS
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Source Baculovirus
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Code CSB-MP363964ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rppH
Uniprot No.
Alternative Names
rppH; nudH; ygdP; b2830; JW2798; RNA pyrophosphohydrolase; EC 3.6.1.-,; Di)nucleoside polyphosphate hydrolase; Ap5A pyrophosphatase
Species
Escherichia coli (strain K12)
Expression Region
1-176
Target Protein Sequence
MIDDDGYRPN VGIVICNRQG QVMWARRFGQ HSWQFPQGGI NPGESAEQAM YRELFEEVGL SRKDVRILAS TRNWLRYKLP KRLVRWDTKP VCIGQKQKWF LLQLVSGDAE INMQTSSTPE FDGWRWVSYW YPVRQVVSFK RDVYRRVMKE FASVVMSLQE NTPKPQNASA YRRKRG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Master regulator of 5'-end-dependent mRNA decay. Accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. Preferentially hydrolyzes diadenosine penta-phosphate with ATP as one of the reaction products. Also able to hydrolyze diadenosine hexa- and tetra-phosphate. Has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP-glucose. In an RNase PH (rph) wild-type strain background, RppH is not required for maturation of tRNAs; however, strains with the truncated rph allele (for example K12 W1485 and its derivatives MG1655 and W3110) require RppH for maturation of certain monocistronic tRNAs with short (<5 nucleotides) leader sequences. In the meningitis causing strain E.coli K1, has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro.
Gene References into Functions
  1. it was also determined that diphosphorylated mRNA decay intermediates are abundant in wild-type E. coli and that their fractional level increases to almost 100% for representative mRNAs in mutant cells lacking RppH. PMID: 29619898
  2. study examined phenotypes of the rppH mutant strain to define the physiological role of RppH in more detail; through various phenotypic analyses of the rppH mutant, found the rppH mutant cells have increased envelope permeability; propose that RppH is associated with regulation of envelope integrity PMID: 28859318
  3. Triphosphate-to-monophosphate conversion to induce 5' end-dependent RNA degradation is a two-step process in E. coli involving gamma-phosphate removal by an unidentified enzyme to enable subsequent beta-phosphate removal by RppH. PMID: 28673541
  4. Data show that the specificity of RNA pyrophosphohydrolase RppH is species-dependent. PMID: 25657006
  5. Data indicate that X-ray structures of RNA pyrophosphohydrolase RppH have revealed molecular features important for RNA recognition. PMID: 25657011
  6. The data suggest that the cellular level of DapF is a critical factor regulating the RppH-catalyzed pyrophosphate removal and the subsequent degradation of target mRNAs. PMID: 25313159
  7. RppH not only catalyses a process functionally reminiscent of eukaryotic mRNA decapping but also bears an evolutionary relationship to the eukaryotic decapping enzyme Dcp2 PMID: 18202662

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Protein Families
Nudix hydrolase family, RppH subfamily
Database Links
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