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Recombinant Escherichia coli Sensor protein KdpD (kdpD), partial

Recombinant Escherichia coli Sensor protein KdpD (kdpD), partial

Code	CSB-YP322039ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP322039ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP322039ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP322039ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP322039ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

kdpD

Uniprot No.

P21865

Alternative Names

kdpD; b0695; JW0683; Sensor protein KdpD; EC 2.7.13.3

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals.

Gene References into Functions

In this report authors provide evidence that the extracellular K(+) concentration serves as one of the stimuli sensed by KdpD. PMID: 23651428
The ArcB and QseC transmembrane (TM) domains are both two-helical motifs, whereas the KdpD TM domain comprises a four-helical bundle with shorter second and third helices. The interhelical distances reveal weak interactions of all three receptors. PMID: 20498088
Data show that kinase activity of KdpD is stimulated in its native membrane environment by fusion with liposomes of anionic, but reduced with liposomes of zwitterionic phospholipids. PMID: 19850005
the sensing and response functions are both located in the C-terminal domain PMID: 16484207
These data clearly show that the extension of the fourth transmembrane helix encompassing the arginine cluster is mainly involved in sensing both K+ limitation and osmotic upshift, which may not be separated mechanistically. PMID: 17704218
Data indicate that stimulus perception by KdpD does not correlate with changes in the cytoplasmic volume, in the intracellular ATP or K(+) concentration. Data propose that a reduction in turgor cannot be the stimulus for KdpD. PMID: 18245296
Dephosphorylated IIANtr enhances activity of the kdp promoter by direct binding of dephosphorylated IIA(Ntr) to the sensor kinase KdpD. PMID: 19400808
The Usp domain within KdpD is important for proper KdpD/KdpE signaling. PMID: 19589130