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Recombinant Escherichia coli Transcriptional regulator gadE (gadE)

Recombinant Escherichia coli Transcriptional regulator gadE (gadE)

Code	CSB-YP351223ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP351223ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP351223ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP351223ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP351223ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

gadE

Uniprot No.

P63204

Alternative Names

gadE; yhiE; yhiT; b3512; JW3480; Transcriptional regulator GadE

Species

Escherichia coli (strain K12)

Expression Region

1-175

Target Protein Sequence

MIFLMTKDSF LLQGFWQLKD NHEMIKINSL SEIKKVGNKP FKVIIDTYHN HILDEEAIKF LEKLDAERII VLAPYHISKL KAKAPIYFVS RKESIKNLLE ITYGKHLPHK NSQLCFSHNQ FKIMQLILKN KNESNITSTL NISQQTLKIQ KFNIMYKLKL RRMSDIVTLG ITSYF

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Regulates the expression of several genes involved in acid resistance. Required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. Binds directly to the 20 bp GAD box found in the control regions of both loci.

Gene References into Functions

These results show how GadEWX simultaneously coordinate many cellular processes to produce the overall response of E. coli to acid stress. PMID: 26258987
These results suggest that overexpression of ArrS positively regulates gadE expression in a post-transcriptional manner. PMID: 24600026
The AraC-family regulators GadX and GadW, previously found to activate gadA/BC in vitro, are now shown in vivo to directly activate gadE expression, which, in turn, activates the gadA/BC genes. PMID: 17660422
The effects of sodium on the acid resistance system of GadA/BC expression appears to be through the modulation of GadX and GadW activity, which in turn controls GadE expression. PMID: 17768258
Three gadE promoters are located within the sensory integration region. PMID: 19220752

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Database Links

KEGG: ecj:JW3480

STRING: 316385.ECDH10B_3688

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