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Recombinant Escherichia coli Uncharacterized symporter ydjN (ydjN), partial

Recombinant Escherichia coli Uncharacterized symporter ydjN (ydjN), partial

Code	CSB-YP304386ENV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP304386ENV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP304386ENV-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP304386ENV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP304386ENV
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ydjN

Uniprot No.

P77529

Alternative Names

ydjN; b1729; JW1718L-cystine transporter YdjN

Species

Escherichia coli (strain K12)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in L-cystine import. Low affinity L-cystine transporter that is mainly involved in L-cystine uptake from outside as a nutrient. Is the primary transporter at high concentrations of cystine. Probably functions as a proton symporter (Probable). Can also transport D-cystine, cysteine and homocystine as a methionine precursor. Mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys). Can also transport S-sulfocysteine and is required for growth on S-sulfocysteine as a sulfur source. Plays the principal role in the 3-mercaptopyruvate sulfurtransferase (3MST)-mediated generation of endogenous hydrogen sulfide (H2S).

Gene References into Functions

study identified and characterized an alternative function of the cystine transporter YdjN in E. coli as a transporter of S-sulfocysteine, a sulfur-containing intermediate in the assimilatory cysteine biosynthesis that is used as a sulfur source for the growth of E. coli PMID: 27481704

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family

Database Links

KEGG: ecj:JW1718

STRING: 316385.ECDH10B_1867

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