Recombinant Female germline-specific tumor suppressor gld-1 (gld-1)

Code CSB-YP623141CXY
MSDS
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Source Yeast
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Code CSB-EP623141CXY
MSDS
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Source E.coli
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Code CSB-EP623141CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP623141CXY
MSDS
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Source Baculovirus
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Code CSB-MP623141CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gld-1
Uniprot No.
Alternative Names
gld-1; T23G11.3Female germline-specific tumor suppressor gld-1; Defective in germ line development protein 1
Species
Caenorhabditis elegans
Expression Region
1-463
Target Protein Sequence
MPSCTTPTYG VSTQLESQSS ESPSRSSVMT PTSLDGDNSP RKRFPIIDNV PADRWPSTRR DGWSSVRAPP PARLTLSTNN RHIMSPISSA YSQTPNSLLS PAMFNPKSRS IFSPTLPATP MSYGKSSMDK SLFSPTATEP IEVEATVEYL ADLVKEKKHL TLFPHMFSNV ERLLDDEIGR VRVALFQTEF PRVELPEPAG DMISITEKIY VPKNEYPDYN FVGRILGPRG MTAKQLEQDT GCKIMVRGKG SMRDKSKESA HRGKANWEHL EDDLHVLVQC EDTENRVHIK LQAALEQVKK LLIPAPEGTD ELKRKQLMEL AIINGTYRPM KSPNPARVMT AVPLLSPTPL RSSGPVLMSP TPGSGLPSTT FGGSILSPTL TASNLLGSNV FDYSLLSPSM FDSFSSLQLA SDLTFPKYPT TTSFVNSFPG LFTSASSFAN QTNTNVSPSG ASPSASSVNN TSF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein which recognizes the 5'-UACUCAU-3' RNA consensus sequence. Binds sequences in both the 5'coding and the 3'-UTR region of rme-2 mRNA. Binds sequences in the 3'-UTR region of cye-1 mRNA. Binds to cyb-2.1, cyb-2.2 and cyb-3 mRNA. Binds sequences in the 3'-UTR region of tra-2 mRNA. Germ line-specific tumor suppressor essential for oogenesis. Controls the spatial pattern of translation of multiple oogenesis specific mRNAs (e.g. yolk receptor rme-2) by repression of translation during early meiotic prophase (leptotene to pachytene) and then derepression of translation during diplotene/ diakinesis, following its degradation. Also functions to promote the male sexual fate in the hermaphrodite germline but not the male germline. Represses translation of the vacuolar ATPase component vha-13 in the distal gonad. Functions redundantly with gld-2 to promote the initiation of meiotic development and/or inhibit stem cell proliferation. By regulating cye-1 expression, prevents entry into mitosis in meiotic germline cells.
Gene References into Functions
  1. GLD-1 expression and degradation during oocyte meiotic progression. PMID: 29496961
  2. glp-1 transcriptional targets lst-1 and sygl-1 are individually required for GLD-1 repression in stem cells; regulation of GLD-1 levels and pattern appears regional in the proliferative zone PMID: 26757772
  3. Gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes. PMID: 26068572
  4. We examine GLD-1-binding motifs among targets conserved in C. elegans and C. briggsae and find that most, but not all, display evidence of shared ancestral binding sites. PMID: 25502909
  5. The study demonstrates that the QUA2 domain distinguishes GLD-1 from other members of the STAR family and contributes more generally to the modulation of RNA-binding affinity and specificity of KH domain containing proteins. PMID: 24838563
  6. Results show the RNA-binding protein GLD-1 in C. elegans affects multiple microRNA pathways and interacts with proteins required for microRNA function. PMID: 24258276
  7. Our results demonstrate that both proteins recognize the glp-1 3' untranslated region (UTR) through adjacent, overlapping binding sites and that POS-1 binding excludes GLD-1 binding PMID: 23034181
  8. we observed that GLD-1 has a widespread role in repressing translation but, importantly, also in stabilizing a sub-population of its mRNA targets PMID: 22693456
  9. Data indicate that the translational repression is mediated by GLD-1, an RNA-binding protein also required for oocyte meiosis and differentiation. PMID: 22106259
  10. GLD-1 is crucial for the CYE-1/CDK-2 mitosis/meiosis control PMID: 21455289
  11. A comprehensive analysis of GLD-1 interaction with mRNA, providing a list of potential GLD-1 targets as well as a quantitative description of the determinants underlying mRNA recognition by GLD-1. PMID: 21169991
  12. GLD-1 binds to TGE RNA and regulates tra-2 translation. PMID: 20573244
  13. Structure of the GLD-1 homodimerization domain: insights into STAR protein-mediated translational regulation PMID: 20223220
  14. Low GLD-1 levels allow proliferation and increased levels promote meiotic entry. PMID: 14660440
  15. identified the consensus STAR-binding element (SBE) required for specific mRNA recognition by GLD-1, a key regulator of germline development PMID: 14718919
  16. Analysis of several mRNA targets containing premature stop codons suggests that in translation repression, GLD-1 either represses ribosome assembly on the target mRNA, or subsequent ribosome elongation to the premature stop codon. PMID: 15105376
  17. GLD-1 mediates a developmental switch in the translational control of C. elegans PAL-1 PMID: 15201219
  18. The RNA binding affinity of 11 mutant GLD-1 proteins was measured, and their binding specificity was assessed with a series of TGE RNAs containing natural or modified nucleotides PMID: 15663930
  19. GLD-1 mediates its repressive effect by directly binding to the 3'UTR of cep-1/p53 mRNA and repressing its translation. PMID: 15707894

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Tissue Specificity
Expressed in proximal and distal oocytes in female worms but is eliminated from proximal oocytes following mating.
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