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Recombinant G-protein regulator 1 (gpr-1), partial

Recombinant G-protein regulator 1 (gpr-1), partial

Code	CSB-YP822115CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP822115CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP822115CXY-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP822115CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP822115CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

gpr-1

Uniprot No.

Q95QJ7

Alternative Names

gpr-1; F22B7.13G-protein regulator 1

Species

Caenorhabditis elegans

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

In the 1-cell embryo, probably together with gpr-2, controls nuclear rotation and spindle elongation during mitosis. Complex of gpr-1 and gpr-2, in association with lin-5, activates G-protein signaling to affect mitotic spindle force. Polarity determinants (par genes) may regulate lin-5/gpr-1/gpr-2/goa-1 locally to create the asymmetric forces that drive spindle movement.

Gene References into Functions

Progeny of these animals (F2) inherit either only F0 maternal or only F0 paternal chromosomes, and thus descend from only either of their grandparents' gametes. PMID: 27479498
GPR-1/2 can sense mechanical properties of the cortex, which may be important for GPR-1/2 function regulating spindle positioning and cytokinesis. PMID: 22927044
Data demonstrate that GPB-1 depletion results in the loss of GPR-1/2 asymmetry during mitosis. PMID: 21652650
Data suggest a mechanism in which RIC-8 favors generation of Galpha free from Gbetagamma and enables GPR-1/2 to mediate asymmetric cell division. PMID: 15479639
Dynamic localization of gpr-1 to cell cortex forces generation of domains during spindle positioning. PMID: 18234174

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Subcellular Location

Cytoplasm, cell cortex. Cytoplasm, cytoskeleton, spindle.

Database Links

KEGG: cel:CELE_F22B7.13

STRING: 6239.F22B7.13

UniGene: Cel.8150

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