Recombinant Guanine nucleotide-binding protein subunit beta-2-like 1 (rack-1)

Code CSB-YP635346CXY
MSDS
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Source Yeast
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Code CSB-EP635346CXY
MSDS
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Source E.coli
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Code CSB-EP635346CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP635346CXY
MSDS
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Source Baculovirus
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Code CSB-MP635346CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rack-1
Uniprot No.
Alternative Names
rack-1; K04D7.1Guanine nucleotide-binding protein subunit beta-2-like 1
Species
Caenorhabditis elegans
Expression Region
2-325
Target Protein Sequence
VQEQMKLTG TLEGHTGWVT QIATYTRNDK TTVLSSSRDK TILVWDVDSV APVDEGPIGR PVRSLTGHNH FVSDVVISSD GQFALSGSWD KTLRLWDLNQ GVSTRQFISH TKDVLSVAFS ADNRQIVSGS RDKSIKLWNT LAQCKYTITD DCHTDWVSTV RFSPSNRDPV IVSAGWDKVV KVWNLGNCRL KTNHIGHTGY VNTVTVSPDG SLCASGGKDG QAMLWDLNEG KHLYTLPGND VINAMSFSPN RYWLCAAVGS SIKIWDLEDK KEIEELKPEI ASSGSSRGSS PQCISLAWSQ DGQTLFAGYT DNIIRVYQVS IRASN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the expression of antimicrobial peptide nlp-29 in response to fungal infection or physical injury.
Gene References into Functions
  1. this study unveils novel defense mechanisms exist for C. elegans in facilitating enhanced immunity by RACK-1 against Shigella flexneri infection PMID: 28502651
  2. Lactobacillus casei pretreatment triggers the TLR mediated and rack-1 dependent p38 MAPK signaling pathway in nematodes. rack-1 is necessary in activating the p38 MAPK pathway by Lactobacillus casei. PMID: 27338631
  3. RACK-1 controls the biogenesis of a subset of miRNAs, including let-7, and in this way plays a role in the heterochronic gene pathway during C. elegans development. PMID: 24776851
  4. RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression. PMID: 21525958
  5. show that depletion of Caenorhabditis elegans RACK-1, which leads to short astral microtubules during prometaphase, specifically affects maintenance of cortical PAR domains and Dynamin localization PMID: 21533050
  6. These studies pinpoint RACK-1 as a component of a novel signaling pathway involving Rac GTPases and UNC-115/abLIM. PMID: 21124943
  7. suggest a mechanism by which RACK-1 directs the dynactin-dependent redistribution of recycling endosomes during the cell cycle PMID: 19158384

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Protein Families
WD repeat G protein beta family, Ribosomal protein RACK1 subfamily
Database Links

KEGG: cel:CELE_K04D7.1

STRING: 6239.K04D7.1.4

UniGene: Cel.4889

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