Recombinant Haloferax volcanii Signal recognition 54 kDa protein (srp54)

Code CSB-YP847287HTU
MSDS
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Source Yeast
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Code CSB-EP847287HTU
MSDS
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Source E.coli
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Code CSB-EP847287HTU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847287HTU
MSDS
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Source Baculovirus
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Code CSB-MP847287HTU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
srp54
Uniprot No.
Alternative Names
srp54; hv54h; HVO_0123; Signal recognition particle 54 kDa protein; SRP54; Hv54h
Species
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Expression Region
1-465
Target Protein Sequence
MVLDNLGSSL RGSLDKLRGK SRLDEDDVQE IVKEIQRSLL SADVDVSLVM DLSDSIKTRA LEEEPPGGTS ARDHVLKIVY EELVDLIGES TEIPLESQTI MLAGLQGSGK TTTSAKMAWW FSKKGLRPAV IQTDTFRPGA YDQAKQMCER AEVDFYGDPD CDDPVQIARE GLEATEDADV HIVDTAGRHA LEDDLIDEIE EIEGVVQPDL NLLVLDAAIG QGAKEQAQQF DESIGIGGVV ITKLDGTAKG GGALTAVNET DSSIAFLGMG ETVQDIERFE PNGFISRLLG MGDLKQLSER VERAMSETQA EDEDWDPEEM MKGNFTLKDM QKQMEAMDKM GPLDQVLDMI PGFGGGIKDQ LPDDAMDVTK DRMRSFEVIM DSMTEEELEN PRKVGASRVR RIAQGSGQDE ETIQELLEQH RMMEQTIKQF QNMGDGDMQR MMKKLQNQGG GGGGGMGGLG GMGPF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.
Subcellular Location
Cytoplasm. Note=The SRP-RNC complex is targeted to the cytoplasmic membrane.
Protein Families
GTP-binding SRP family, SRP54 subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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