Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Microbiology
Alternative Names
cagA; caiCytotoxicity-associated immunodominant antigen; 120 kDa protein
Species
Helicobacter pylori (Campylobacter pylori)
Expression Region
918-1147aa
Target Protein Sequence
KVNAKIDRLNQIASGLGVVGQAAGFPLKRHDKVDDLSKVGLSRNQELAQKIDNLNQAVSEAKAGFFGNLEQTIDKLKDSTKHNPMNLWVESAKKVPASLSAKLDNYATNSHIRINSNIKNGAINEKATGMLTQKNPEWLKLVNDKIVAHNVGSVPLSEYDKIGFNQKNMKDYSDSFKFSTKLNNAVKDTNSGFTQFLTNAFSTASYYCLARENAEHGIKNVNTKGGFQKS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Helicobacter pylori cagA was expressed with the amino acid range of 918-1147. This cagA protein is expected to have a theoretical molecular weight of 41.3 kDa. This cagA recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of cagA, which enables a simple process of detecting and purifying the cagA recombinant protein in the following steps.
The Helicobacter pylori cytotoxicity-associated immunodominant antigen (cagA) is a crucial virulence factor associated with certain strains of H. pylori, a bacterium that infects the human stomach. CagA is delivered into gastric epithelial cells by a type IV secretion system (T4SS), which is encoded by the cag pathogenicity island (cagPAI). Once inside the host cell, CagA undergoes phosphorylation by host cell kinases, leading to alterations in various cellular signaling pathways. Phosphorylated CagA has been linked to disruption of cell polarity, cell-cell adhesion, and the induction of pro-inflammatory responses. Additionally, chronic infection with cagA-positive H. pylori strains is associated with an increased risk of developing peptic ulcers and gastric cancer. The molecular mechanisms by which CagA influences host cell processes are complex and involve its interactions with various host cell proteins. Understanding the role of CagA in H. pylori pathogenesis is crucial for developing strategies to manage and prevent associated diseases.
