Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ORF2

Uniprot No.

Q68985

Research Area

Microbiology

Alternative Names

ORF2Secreted protein ORF2; Protein ORF2; pORF2

Species

Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad) (HEV-1)

Source

Baculovirus

Expression Region

20-660aa

Target Protein Sequence

PPPGQPSGRRRGRRSGGSGGGFWGDRVDSQPFAIPYIHPTNPFAPNVTAAAGAGPRVRQPVRPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLSTSPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVARATIRYRPLVPNAVGGYAISISFWPQTTPTPTSVDMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIHGSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFMKDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQQDKGIAIPNDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQYSKIFFVLPLRGKLSFWEAGTTRPGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLGPHSALALLEDTLDYPARAHTFDDFCPECRPLGLQGCAFQSTVAELQRLKMKVGKTREL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

71.3 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Hepatitis E virus genotype 1 ORF2 covers amino acids 20-660. The theoretical molecular weight of the ORF2 protein is 71.3 kDa. Expression of this ORF2 protein is conducted in baculovirus. The N-terminal 10xHis tag was fused into the coding gene segment of ORF2, making it easier to detect and purify the ORF2 recombinant protein in the later stages of expression and purification.

Research on the pro-secreted protein ORF2 primarily focuses on revealing its secretion function and potential roles in diseases. Firstly, the protein encoded by ORF2 is believed to possess secretion functionality, potentially executing crucial biological tasks outside the cells, prompting scientists' interest in its involvement in intercellular communication and immune regulation. Secondly, researchers are dedicated to uncovering the associations between ORF2 and various diseases, including cancer and immune-related disorders, for a better understanding of its exact roles in the occurrence and development of these conditions.

Target Background

Function

Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.; Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation. Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes.

Subcellular Location

[Isoform Secreted protein ORF2]: Secreted.; [Isoform Capsid protein ORF2]: Virion. Host cytoplasm. Host endoplasmic reticulum. Host Golgi apparatus. Host cell surface.

Protein Families

Hepevirus capsid protein family

Code	CSB-BP727851HVZ
Abbreviation	Recombinant Hepatitis E virus genotype 1 ORF2 protein, partial
MSDS	MSDS Datasheet
Size	$472
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Hepatitis E virus genotype 1 Secreted protein ORF2 (ORF2), partial

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