Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ORF2

Uniprot No.

Q68985

Research Area

Others

Alternative Names

ORF2Secreted protein ORF2; Protein ORF2; pORF2

Species

Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad) (HEV-1)

Source

E.coli

Expression Region

20-660aa

Target Protein Sequence

PPPGQPSGRRRGRRSGGSGGGFWGDRVDSQPFAIPYIHPTNPFAPNVTAAAGAGPRVRQPVRPLGSAWRDQAQRPAAASRRRPTTAGAAPLTAVAPAHDTPPVPDVDSRGAILRRQYNLSTSPLTSSVATGTNLVLYAAPLSPLLPLQDGTNTHIMATEASNYAQYRVARATIRYRPLVPNAVGGYAISISFWPQTTPTPTSVDMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIHGSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYSSTARHRLRRGADGTAELTTTAATRFMKDLYFTSTNGVGEIGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSRPVVSANGEPTVKLYTSVENAQQDKGIAIPNDIDLGESRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQSTYGSSTGPVYVSDSVTLVNVATGAQAVARSLDWTKVTLDGRPLSTIQQYSKIFFVLPLRGKLSFWEAGTTRPGYPYNYNTTASDQLLVENAAGHRVAISTYTTSLGAGPVSISAVAVLGPHSALALLEDTLDYPARAHTFDDFCPECRPLGLQGCAFQSTVAELQRLKMKVGKTREL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

84.8kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To prepare this Recombinant HEV-1 ORF2 protein, the recombinant DNA was required, which was generated by fusing the ORF2 gene with N-terminal 6xHis-SUMO tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this ORF2 protein production. After purification, a premium ORF2 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 90%+.

ORF2 is a protein encoding gene that provides an instruction in making a protein named secreted protein ORF2 or protein ORF2. In Hepatitis E virus (HEV), the protein encoded by ORF2 gene has two isoforms, secreted protein ORF2 and capsid protein ORF2. The sequence difference between isoform 2 and the isoform 1 is lack of the 1-15 amino acid sequence. The difference between the sequence directly determines their different functions. Isoform 1 can inhibit host antibody-mediated neutralization without blocking viral cell entry. Isoform 2 is the major virion component to form the viral capsid. ORF2 from HEV plays multiple functions in both viral replication and pathogenesis.

Target Background

Function

Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.; Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation. Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes.

Subcellular Location

[Isoform Secreted protein ORF2]: Secreted.; [Isoform Capsid protein ORF2]: Virion. Host cytoplasm. Host endoplasmic reticulum. Host Golgi apparatus. Host cell surface.

Protein Families

Hepevirus capsid protein family

Code	CSB-EP727851HVZ
Abbreviation	Recombinant Hepatitis E virus genotype 1 ORF2 protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Hepatitis E virus genotype 1 Secreted protein ORF2 (ORF2), partial

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