Recombinant Histone-lysine N-methyltransferase mes-4 (mes-4), partial

Code CSB-YP882047CXY
MSDS
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Source Yeast
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Code CSB-EP882047CXY
MSDS
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Source E.coli
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Code CSB-EP882047CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882047CXY
MSDS
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Source Baculovirus
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Code CSB-MP882047CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mes-4
Uniprot No.
Alternative Names
mes-4; Y2H9A.1; Histone-lysine N-methyltransferase mes-4; EC 2.1.1.357; Maternal-effect sterile protein 4
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Histone methyltransferase. Dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Plays a central role in early development and is responsible for all H3 'Lys-36' dimethylation until about the 40-cell stage. Indirectly involved in the global inactivation of the X chromosomes in germline cells, possibly by excluding the mes-2-mes-3-mes-6 repressive Polycomb complex from the autosomes. Not related to transcription elongation. Required for small-RNA-induced H3K27 trimethylation. May suppress sensitivity to RNAi. May regulate the expression of genes required for vulval development.
Gene References into Functions
  1. MES-4 transmits the memory of gene expression in the parental germ line to offspring. PMID: 20824077
  2. MES-4 has histone H3 methyltransferase (HMT) activity in vitro, and is required for histone H3K36 dimethylation in mitotic and early meiotic germline nuclei and early embryos. PMID: 16968818
Subcellular Location
Nucleus. Chromosome. Note=Specifically associated with the autosomes and with the distal tip of chromosome X. Colocalizes with methylated 'Lys-4' of histone H3.
Protein Families
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily
Tissue Specificity
In adults, it is predominantly expressed in the germline, and weakly expressed in intestinal cells.
Database Links

KEGG: cel:CELE_Y2H9A.1

STRING: 6239.Y2H9A.1.2

UniGene: Cel.6195

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