Recombinant Homeobox protein unc-30 (unc-30)

Code CSB-YP345238CXY
MSDS
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Source Yeast
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Code CSB-EP345238CXY
MSDS
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Source E.coli
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Code CSB-EP345238CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345238CXY
MSDS
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Source Baculovirus
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Code CSB-MP345238CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
unc-30
Uniprot No.
Alternative Names
unc-30; B0564.10; Homeobox protein unc-30; Uncoordinated protein 30
Species
Caenorhabditis elegans
Expression Region
1-323
Target Protein Sequence
MDDNTATLTA IHQQQQSHNR FSNPLVCVGQ LDHHSLLPEH SISSSLAPLT HNPYAFNYSI PLPPTDITTK LPKLELLSLD VKQEQDDNHL DTSSPTDSTG NGSTNGGKIQ KPRRQRTHFT SHQLTELENW FSRNRYPDMA CREEIAVWIS LTEPRVRVWF KNRRAKWRKR ERNYVIDNGQ GTTKVTAQSL DPLGSLQNTF PQTLLQSSSS QLDDSAVTSS SFYGYGGAWQ QNPYYSRNNQ TTFNWQIKPQ DQFQFQTIPM SPTTATSRFS TAANLAPLPT AQAAFSTSAT SSNDKLKLMD GLSNSLSSSL GQPYQPCQYS GPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional regulator within D-type neurons that controls terminal differentiation. Required for the development and function of the 19 inhibitory GABAergic (gamma-aminobutyric-acid-secreting) D-type motor neurons, which control locomotion. Plays a role in regulating synapse formation in dorsal D (DD) and ventral D (VD) GABAergic motor neurons, possibly in part by regulating the expression of the neural regulator oig-1. Also required for expression of flp-13 in DD motor neurons. Plays a role in respecification of DD motor neurons through regulation of genes involved in the modulation of cellular cAMP including pde-4 which hydrolyzes cAMP and acy-1 which catalyzes cAMP formation.
Gene References into Functions
  1. This work provides the first example of genetic redundancy between the related yet evolutionarily distant OTX and PITX families of bicoid class homeodomain factors and demonstrates the power of quantitative developmental phenotyping in C. elegans PMID: 25738873
Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
Expressed in D-type GABAergic motor neurons at high levels in young larvae, in which the motor circuitry is formed, and at low levels in older animals. Also present in six non-GABAergic motor neurons and is absent from the seven non-D-type GABAergic neuro
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