Recombinant Horse Interleukin-10(IL10)

Code CSB-YP634667HO
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Source Yeast
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Code CSB-EP634667HO
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Source E.coli
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Code CSB-EP634667HO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP634667HO
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Source Baculovirus
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Code CSB-MP634667HO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names IL10
Uniprot No. Q28374
Alternative Names IL10Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF
Species Equus caballus (Horse)
Expression Region 19-178
Target Protein Sequence SR DRGTQSENSC THFPTSLPHM LHELRAAFSR VKTFFQMKDQ LDNMLLNGSL LEDFKGYLGC QALSEMIQFY LEEVMPQAEN HGPDIKEHVN SLGEKLKTLR VRLRRCHRFL PCENKSKAVE QVKSAFSKLQ EKGVYKAMSE FDIFINYIEA YMTTKMKN
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Gene References into Functions
  1. The ability of an EHV-1 isolate to down-regulate IL-10 production might contribute to increased local inflammation and a higher risk for neurological manifestation of the disease after infection with Ab4. PMID: 21764140
  2. The data suggested in foals there is an impaired Th2 response, the immune response is Th1 biased, interferon-gamma production is qualitatively similar to adult horses, and regulatory IL-10 production by T cells is mature. PMID: 20374696
  3. serum IL-6:IL-10 ratio is likely to provide a valuable prognosticator for neonatal septicemia PMID: 19501415
  4. The effect of clenbuterol on cytokine metabolism in the peripheral white blood cells in those horses with small airway diseases that have been exposed to lipopolysaccharides is reported. PMID: 17139543
  5. The contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of IL-6, IL-10 and TNF-alpha, in horses with recurrent airway obstruction during exacerbation and in remission is reported. PMID: 18294392

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Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: ecb:100034187

STRING: 9796.ENSECAP00000007097

UniGene: Eca.13090

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