Recombinant Human Gamma-crystallin B (CRYGB)

Code CSB-EP006018HU
MSDS
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006018HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CRYGB.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006018HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CRYGB.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
CRYGB
Uniprot No.
Research Area
Neuroscience
Alternative Names
CRYGB; CRYG2Gamma-crystallin B; Gamma-B-crystallin; Gamma-crystallin 1-2
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-175aa
Target Protein Sequence
MGKITFYEDRAFQGRSYECTTDCPNLQPYFSRCNSIRVESGCWMIYERPNYQGHQYFLRRGEYPDYQQWMGLSDSIRSCCLIPPHSGAYRMKIYDRDELRGQMSELTDDCISVQDRFHLTEIHSLNVLEGSWILYEMPNYRGRQYLLRPGEYRRFLDWGAPNAKVGSLRRVMDLY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
24.9 kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Crystallins are the dominant structural components of the vertebrate eye lens.
Gene References into Functions
  1. Glycation of human gammaB-crystallin PMID: 28013006
  2. Allelic variant frequency of the gamma-crystallin promoter (g(-47) ->a) affects the level of its expression in platelets from cataract patients. PMID: 26552302
  3. Complex heterogeneous mutations in the gammaB crystallin gene have been described resulting in autosomal dominant congenital cataracts with three distinct phenotypes (lamellar, anterior polar, and complete cataracts) in the same family. PMID: 23288985
  4. -47C allele of rs2289917 in CRYGB showed the strongest association with cataract. PMID: 21941057
  5. The alphaB-crystallin oligomers formed long-lived stable complexes with their gammaD-crystallin substrates. PMID: 20621668
  6. In gammaD-crystallin, methylation is exclusively at Cys 110, whereas in gammaC- and gammaB-crystallins, the principal methylation site is Cys 22 with minor methylation at Cys 79 PMID: 12876325
  7. Possible sites for posttranslational modifications in gamma B crystallin. PMID: 14517968

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Involvement in disease
Cataract 39, multiple types (CTRCT39)
Protein Families
Beta/gamma-crystallin family
Database Links

HGNC: 2409

OMIM: 123670

KEGG: hsa:1419

STRING: 9606.ENSP00000260988

UniGene: Hs.248102

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