Recombinant Human 60S ribosomal protein L3 (RPL3), partial

Code CSB-RP023854h
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
RPL3
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B; HIV1 TAR RNA binding protein B; MGC103388; MGC104284; MGC72934; Ribosomal protein L3; Ribosomal protein L3, isoform a; RL3_HUMAN; rpl3; TARBP B; TARBP-B
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-247aa
Target Protein Sequence
SHRKFSAPRHGSLGFLPRKRSSRHRGKVKSFPKDDPSKPVHLTAFLGYKAGMTHIVREVDRPGSKVNKKEVVEAVTIVETPPMVVVGIVGYVETPRGLRTFKTVFAEHISDECKRRFYKNWHKSKKKAFTKYCKKWQDEDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
55.4kDa
Protein Length
Partial
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The L3 protein is a component of the large subunit of cytoplasmic ribosomes.
Gene References into Functions
  1. The sensitivity of p53 null colon cancer cells to 5-FU can be enhanced using proapoptotic rpL3 gene. PMID: 27835895
  2. nucleolar stress induced by 5-fluorouracil in colon cancer cells devoid of p53 leads to the activation of ribosomal protein L3 as proapoptotic factor PMID: 27385096
  3. We found that uL3 down-regulation correlates positively with multidrug resistance. Restoration of the uL3 protein level re-sensitized the resistant cells to the drug by regulating the reactive oxygen species (ROS) levels, glutathione content, glutamate release, and cystine uptake. PMID: 28273808
  4. rpL3 participates in the cell response to chemotherapy acting as a critical regulator of cell cycle, apoptosis and DNA repair, by modulating p21 expression PMID: 25473889
  5. The rpL3 is able to modulate the protein amounts of p21 through a novel pathway independent from p53 involving Sp1 and NPM. PMID: 23255119
  6. NPM and KHSRP are two newly identified proteins involved in the regulation of rpL3 gene expression via alternative pre-mRNA splicing. PMID: 21705779
  7. working model in which rpL3 recruits hnRNP H1 and, through cooperation with other splicing factors, promotes selection of the alternative splice site PMID: 20100605

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Subcellular Location
Nucleus, nucleolus. Cytoplasm.
Protein Families
Universal ribosomal protein uL3 family
Database Links

HGNC: 10332

OMIM: 604163

KEGG: hsa:6122

STRING: 9606.ENSP00000346001

UniGene: Hs.119598

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