Recombinant Human 7SK snRNA methylphosphate capping enzyme (MEPCE)

Code CSB-YP762569HU
MSDS
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Source Yeast
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Code CSB-EP762569HU
MSDS
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Source E.coli
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Code CSB-EP762569HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP762569HU
MSDS
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Source Baculovirus
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Code CSB-MP762569HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MEPCE
Uniprot No.
Alternative Names
MEPCE; BCDIN37SK snRNA methylphosphate capping enzyme; MePCE; EC 2.1.1.-; Bicoid-interacting protein 3 homolog; Bin3 homolog
Species
Homo sapiens (Human)
Expression Region
1-689
Target Protein Sequence
MIEMAAEKEP FLVPAPPPPL KDESGGGGGP TVPPHQEAAS GELRGGTERG PGRCAPSAGS PAAAVGRESP GAAATSSSGP QAQQHRGGGP QAQSHGEARL SDPPGRAAPP DVGEERRGGG GTELGPPAPP RPRNGYQPHR PPGGGGGKRR NSCNVGGGGG GFKHPAFKRR RRVNSDCDSV LPSNFLLGGN IFDPLNLNSL LDEEVSRTLN AETPKSSPLP AKGRDPVEIL IPKDITDPLS LNTCTDEGHV VLASPLKTGR KRHRHRGQHH QQQQAAGGSE SHPVPPTAPL TPLLHGEGAS QQPRHRGQNR DAPQPYELNT AINCRDEVVS PLPSALQGPS GSLSAPPAAS VISAPPSSSS RHRKRRRTSS KSEAGARGGG QGSKEKGRGS WGGRHHHHHP LPAAGFKKQQ RKFQYGNYCK YYGYRNPSCE DGRLRVLKPE WFRGRDVLDL GCNVGHLTLS IACKWGPSRM VGLDIDSRLI HSARQNIRHY LSEELRLPPQ TLEGDPGAEG EEGTTTVRKR SCFPASLTAS RGPIAAPQVP LDGADTSVFP NNVVFVTGNY VLDRDDLVEA QTPEYDVVLC LSLTKWVHLN WGDEGLKRMF RRIYRHLRPG GILVLEPQPW SSYGKRKTLT ETIYKNYYRI QLKPEQFSSY LTSPDVGFSS YELVATPHNT SKGFQRPVYL FHKARSPSH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it. Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex. MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center.
Gene References into Functions
  1. The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRNA), MePCE, and Larp7, also functions as a canonical transcription factor that, in collaboration with the little elongation complex (LEC) comprising ELL, Ice1, Ice2, and ZC3H8, promotes transcription of RNAPII-specific spliceosomal snRNA and small nucleolar RNA (snoRNA) genes. PMID: 28254838
  2. Data indicate the binding of RNA-binding protein HEXIM with 7SKsnRNP complex comprising the non-coding RNA 7SK and proteins MePCE and LARP7. PMID: 25863285
  3. Capping enzyme can play a direct role in transcription by modulating displacement of nascent RNA during transcription. PMID: 17978174
Subcellular Location
Nucleus.
Protein Families
Methyltransferase superfamily
Tissue Specificity
Expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. Weakly or not expressed in other tissues.
Database Links

HGNC: 20247

OMIM: 611478

KEGG: hsa:56257

STRING: 9606.ENSP00000308546

UniGene: Hs.178011

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