Recombinant Human ARF GTPase-activating protein GIT2 (GIT2)

Code CSB-YP613489HU
MSDS
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Source Yeast
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Code CSB-EP613489HU
MSDS
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Source E.coli
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Code CSB-EP613489HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613489HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP613489HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GIT2
Uniprot No.
Alternative Names
ARF GAP GIT2; ARF GTPase activating protein GIT2; ARF GTPase-activating protein GIT2; CAT 2; CAT-2; CAT2; Cool associated tyrosine phosphorylated protein 2; Cool interacting tyrosine phosphorylated protein 2; Cool-interacting tyrosine-phosphorylated protein 2; DKFZp686G01261; G protein coupled receptor kinase interacting ArfGAP 2; G protein coupled receptor kinase interactor 2; G protein-coupled receptor kinase-interactor 2; GIT 2; GIT2; GIT2_HUMAN; GRK interacting protein 2; GRK-interacting protein 2; KIAA0148; MGC760
Species
Homo sapiens (Human)
Expression Region
1-759
Target Protein Sequence
MSKRLRSSEV CADCSGPDPS WASVNRGTFL CDECCSVHRS LGRHISQVRH LKHTPWPPTL LQMVETLYNN GANSIWEHSL LDPASIMSGR RKANPQDKVH PNKAEFIRAK YQMLAFVHRL PCRDDDSVTA KDLSKQLHSS VRTGNLETCL RLLSLGAQAN FFHPEKGNTP LHVASKAGQI LQAELLAVYG ADPGTQDSSG KTPVDYARQG GHHELAERLV EIQYELTDRL AFYLCGRKPD HKNGQHFIIP QMADSSLDLS ELAKAAKKKL QSLSNHLFEE LAMDVYDEVD RRETDAVWLA TQNHSALVTE TTVVPFLPVN PEYSSTRNQG RQKLARFNAH EFATLVIDIL SDAKRRQQGS SLSGSKDNVE LILKTINNQH SVESQDNDQP DYDSVASDED TDLETTASKT NRQKSLDSDL SDGPVTVQEF MEVKNALVAS EAKIQQLMKV NNNLSDELRI MQKKLQTLQS ENSNLRKQAT TNVYQVQTGS EYTDTSNHSS LKRRPSARGS RPMSMYETGS GQKPYLPMGE ASRPEESRMR LQPFPAHIGR SALVTSSSSL PSFPSTLSWS RDESARRASR LEKQNSTPES DYDNTPNDME PDGMGSSRKG RQRSMVWPGD GLVPDTAEPH VAPSPTLPST EDVIRKTEQI TKNIQELLRA AQENKHDSYI PCSERIHVAV TEMAALFPKK PKSDMVRTSL RLLTSSAYRL QSECKKTLPG DPGSPTDVQL VTQQVIQCAY DIAKAAKQLV TITTKENNN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase-activating protein for ADP ribosylation factor family members, including ARF1.
Gene References into Functions
  1. our work identifies DOCK5 as a key regulator of epithelial invasion and metastasis, and demonstrates that suppression of DOCK5 by GIT2 represents a previously unappreciated mechanism for coordination of Rho and Rac GTPases. PMID: 27669437
  2. Short-term EGF stimulation if of lung tumor cells can increase the interaction between RUSC2 and GIT2, prolonged stimulation leads to a decrease of their interaction through activating Rab35. PMID: 27238570
  3. GIT2 plays an important role in MRE11/ATM/H2AX-mediated DNA damage responses. PMID: 25605334
  4. ADMA and L-arginine are substrates of human CAT2A, CAT2B, OCT2 and MATE1. Transport kinetics of CAT2A, CAT2B, and OCT2 indicate a low affinity, high capacity transport, which may be relevant for renal and hepatic elimination of ADMA or L-arginine PMID: 23864433
  5. Zeb1 is regulated by the Arf GTPase-activating protein (GAP) Git2. PMID: 23591815
  6. GIT2 protein is tightly associated with PIX family Rac1/Cdc42 guanine nucleotide exchange factors as a multimeric nexus capable of linking together important signaling molecules. PMID: 15212761
  7. The expression level of lymphocyte GRK might show the severity of CHF, and ACEI treatment could reduce the level of GRK in CHF patients. PMID: 16501306
  8. GTI proteins regulate cytoskeletal dynamics by feedback inhibition of Rac1, they participate in receptor internalization by regulating membrane trafficking between PMID: 16598076
  9. These results suggest that inactivation of GIT2 function is a required step for induction of cell motility and that GIT2 may be a target of oncogenic signaling pathways that regulate cell migration. PMID: 16628223
  10. Git2-short, weakly interacting with paxillin, functions in the regulation of Golgi organization, actin cytoskeletal organization, and subcellular localization of paxillin, important in integrin-mediated cell adhesion and intracellular signaling. PMID: 11251077

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Database Links

HGNC: 4273

OMIM: 608564

KEGG: hsa:9815

STRING: 9606.ENSP00000347464

UniGene: Hs.434996

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