Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
ACAT 1; ACAT; acat1; Acetoacetyl CoA thiolase; acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; Acetyl CoA acetyltransferase; mitochondrial ; Acetyl Coenzyme A acetyltransferase 1; Acetyl-CoA acetyltransferase; acetyl-coa acetyltransferase precursor; mitochondrial ; Acetyl-CoA thiolase; mitochondrial; acetyl-Coenzyme A acetyltransferase 1; MAT; mitochondrial acetoacetyl-CoA thiolase; mitochondrial; RATACAL; T2; testicular tissue protein Li 198; THIL; THIL_HUMAN
Species
Homo sapiens (Human)
Expression Region
34-427aa
Target Protein Sequence
VSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQKL
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Human ACAT1 covers amino acids 34-427. The calculated molecular weight for this ACAT1 protein is 45.0kDa. The ACAT1 protein was expressed in e.coli. The N-terminal 10xHis tag was fused into the coding gene segment of ACAT1, making it easier to detect and purify the ACAT1 recombinant protein in the later stages of expression and purification.
Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a mitochondrial protein primarily associated with lipid metabolism and energy regulation. In lipid metabolism, ACAT1 serves as a crucial enzyme in the acetyl-coenzyme A metabolic pathway, catalyzing the transfer of acetyl-coenzyme A and participating in cholesterol synthesis and ketone body generation. This positions ACAT1 to play a vital role in regulating cholesterol levels and maintaining energy balance. Recent research indicates that ACAT1 is also associated with various diseases such as metabolic disorders and neurological conditions, sparking widespread interest among scientists.