Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ACAT1

Uniprot No.

P24752

Research Area

Metabolism

Alternative Names

ACAT 1; ACAT; acat1; Acetoacetyl CoA thiolase; acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; Acetyl CoA acetyltransferase; mitochondrial ; Acetyl Coenzyme A acetyltransferase 1; Acetyl-CoA acetyltransferase; acetyl-coa acetyltransferase precursor; mitochondrial ; Acetyl-CoA thiolase; mitochondrial; acetyl-Coenzyme A acetyltransferase 1; MAT; mitochondrial acetoacetyl-CoA thiolase; mitochondrial; RATACAL; T2; testicular tissue protein Li 198; THIL; THIL_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

34-427aa

Target Protein Sequence

VSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQKL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

45.0kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human ACAT1 covers amino acids 34-427. The calculated molecular weight for this ACAT1 protein is 45.0kDa. The ACAT1 protein was expressed in e.coli. The N-terminal 10xHis tag was fused into the coding gene segment of ACAT1, making it easier to detect and purify the ACAT1 recombinant protein in the later stages of expression and purification.

Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a mitochondrial protein primarily associated with lipid metabolism and energy regulation. In lipid metabolism, ACAT1 serves as a crucial enzyme in the acetyl-coenzyme A metabolic pathway, catalyzing the transfer of acetyl-coenzyme A and participating in cholesterol synthesis and ketone body generation. This positions ACAT1 to play a vital role in regulating cholesterol levels and maintaining energy balance. Recent research indicates that ACAT1 is also associated with various diseases such as metabolic disorders and neurological conditions, sparking widespread interest among scientists.

Target Background

Function

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.

Gene References into Functions

High ACAT1 expression is associated with breast cancer. PMID: 30092298
Insulin promotes progression of colon cancer by upregulation of ACAT1. PMID: 29793481
ACAT1 exonic mutations that affect ESE sequences may result in aberrant splicing. This may affect the activity of mitochondrial acetoacetyl-CoA thiolase. PMID: 27748876
compound heterozygous of ACAT1 gene mutations probably underlie the beta-ketothiolase deficiency in our patient PMID: 27264805
Data indicate that acetyl-CoA acetyltransferase (ACAT1) and malate dehydrogenase (MDH2) are involved in various drug-resistance-forming mechanisms. PMID: 25639359
the pyruvate dehydrogenase complex is regulated by Tyr phosphorylation of PDP1, which toggles recruitment between ACAT1 and SIRT3 PMID: 24486017
these findings indicate that ACAT1 expression could serve as a potential prognostic marker in prostate cancer, specifically in differentiating indolent and aggressive forms of cancer. PMID: 24311408
ACAT1 expression is substantially elevated in human castration-resistant metastatic prostate cancer tissues. PMID: 23443136
Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085
We herein identified a common mutation, R208X, in Vietnamese patients. We identified R208X homozygously in six patients and heterozygously in two patients among eight Vietnamese patients. PMID: 20156697
the siblings with the same T2 gene mutations present different clinical severity of T2 deficiency PMID: 20046049
Single base substitutions at the initiator codon produced wild-type T2 polypeptide to various degrees PMID: 12754704
mild mutations (A132G, D339-V340insD) retained some residual T2 activity PMID: 15128923
Cloning and sequencing long range PCR products revealed a 6.4kb deletion. Alu element-mediated unequal homologous recombination between an Alu-Sx in intron 1 and another Alu-Y in intron 4 appears to be responsible for this deletion. PMID: 16935016
E252del is a temperature-sensitive K(m) mutant. PMID: 17236799
Crystallographic and kinetic studies were made on mitochondrial acetyl-CoA thiolase: the importance of potassium and chloride ions were noted for its structure and function. PMID: 17371050
A point mutation was responsible for the aberrant RNA splicing of the mitochondrial acetoacetyl-CoA thiolase gene. PMID: 18511318
sequence recognition by the catalytic domain differs between hT2 and hT10 in that hT10 requires a pre-existing GalNAc residue while hT2 does not PMID: 18562306
I/D polymorphism of ACE gene and AC AT1 gene influence the development of hypertension and Left Ventricular Hypertrophy in Hemodialysis patients. PMID: 19112833

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Involvement in disease

3-ketothiolase deficiency (3KTD)

Subcellular Location

Mitochondrion.

Protein Families

Thiolase family

Database Links

HGNC: 93

OMIM: 203750

KEGG: hsa:38

STRING: 9606.ENSP00000265838

UniGene: Hs.232375

Code	CSB-EP001134HU
Abbreviation	Recombinant Human ACAT1 protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Acetyl-CoA acetyltransferase, mitochondrial (ACAT1)

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