ACAT1 Antibody

Code CSB-PA001134LA01HU
Size US$299 How to order?
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Rat liver tissue, Mouse heart tissue, Mouse liver tissue Mouse kindey tissue
    All lanes: ACAT1 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46, 18 kDa
    Observed band size: 46 kDa

  • Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA001134LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001134LA01HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ACAT1 Polyclonal antibody
Uniprot No. P24752
Target Names ACAT1
Alternative Names ACAT 1 antibody; ACAT antibody; acat1 antibody; Acetoacetyl CoA thiolase antibody; acetoacetyl Coenzyme A thiolase antibody; Acetoacetyl-CoA thiolase antibody; Acetyl CoA acetyltransferase; mitochondrial antibody; Acetyl Coenzyme A acetyltransferase 1 antibody; Acetyl-CoA acetyltransferase antibody; acetyl-coa acetyltransferase precursor; mitochondrial antibody; Acetyl-CoA thiolase; mitochondrial antibody; acetyl-Coenzyme A acetyltransferase 1 antibody; MAT antibody; mitochondrial acetoacetyl-CoA thiolase antibody; mitochondrial antibody; RATACAL antibody; T2 antibody; testicular tissue protein Li 198 antibody; THIL antibody; THIL_HUMAN antibody
Raised in Rabbit
Species Reactivity Human, Mouse, Rat
Immunogen Recombinant Human Acetyl-CoA acetyltransferase, mitochondrial protein (76-106AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The ACAT1 Antibody (Product code: CSB-PA001134LA01HU) is Non-conjugated. For ACAT1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA001134LB01HU ACAT1 Antibody, HRP conjugated ELISA
FITC CSB-PA001134LC01HU ACAT1 Antibody, FITC conjugated
Biotin CSB-PA001134LD01HU ACAT1 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
Gene References into Functions
  1. High ACAT1 expression is associated with breast cancer. PMID: 30092298
  2. Insulin promotes progression of colon cancer by upregulation of ACAT1. PMID: 29793481
  3. ACAT1 exonic mutations that affect ESE sequences may result in aberrant splicing. This may affect the activity of mitochondrial acetoacetyl-CoA thiolase. PMID: 27748876
  4. compound heterozygous of ACAT1 gene mutations probably underlie the beta-ketothiolase deficiency in our patient PMID: 27264805
  5. Data indicate that acetyl-CoA acetyltransferase (ACAT1) and malate dehydrogenase (MDH2) are involved in various drug-resistance-forming mechanisms. PMID: 25639359
  6. the pyruvate dehydrogenase complex is regulated by Tyr phosphorylation of PDP1, which toggles recruitment between ACAT1 and SIRT3 PMID: 24486017
  7. these findings indicate that ACAT1 expression could serve as a potential prognostic marker in prostate cancer, specifically in differentiating indolent and aggressive forms of cancer. PMID: 24311408
  8. ACAT1 expression is substantially elevated in human castration-resistant metastatic prostate cancer tissues. PMID: 23443136
  9. Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines. PMID: 21791085
  10. We herein identified a common mutation, R208X, in Vietnamese patients. We identified R208X homozygously in six patients and heterozygously in two patients among eight Vietnamese patients. PMID: 20156697
  11. the siblings with the same T2 gene mutations present different clinical severity of T2 deficiency PMID: 20046049
  12. Single base substitutions at the initiator codon produced wild-type T2 polypeptide to various degrees PMID: 12754704
  13. mild mutations (A132G, D339-V340insD) retained some residual T2 activity PMID: 15128923
  14. Cloning and sequencing long range PCR products revealed a 6.4kb deletion. Alu element-mediated unequal homologous recombination between an Alu-Sx in intron 1 and another Alu-Y in intron 4 appears to be responsible for this deletion. PMID: 16935016
  15. E252del is a temperature-sensitive K(m) mutant. PMID: 17236799
  16. Crystallographic and kinetic studies were made on mitochondrial acetyl-CoA thiolase: the importance of potassium and chloride ions were noted for its structure and function. PMID: 17371050
  17. A point mutation was responsible for the aberrant RNA splicing of the mitochondrial acetoacetyl-CoA thiolase gene. PMID: 18511318
  18. sequence recognition by the catalytic domain differs between hT2 and hT10 in that hT10 requires a pre-existing GalNAc residue while hT2 does not PMID: 18562306
  19. I/D polymorphism of ACE gene and AC AT1 gene influence the development of hypertension and Left Ventricular Hypertrophy in Hemodialysis patients. PMID: 19112833

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Involvement in disease 3-ketothiolase deficiency (3KTD)
Subcellular Location Mitochondrion.
Protein Families Thiolase family
Database Links

HGNC: 93

OMIM: 203750

KEGG: hsa:38

STRING: 9606.ENSP00000265838

UniGene: Hs.232375

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