Recombinant Human Alpha-1A adrenergic receptor(ADRA1A),partial

Code CSB-YP001385HU1
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Source Yeast
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Code CSB-EP001385HU1
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Source E.coli
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Code CSB-EP001385HU1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001385HU1
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Source Baculovirus
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Code CSB-MP001385HU1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ADRA1A
Uniprot No. P35348
Alternative Names ADRA1A; ADRA1C; Alpha-1A adrenergic receptor; Alpha-1A adrenoreceptor; Alpha-1A adrenoceptor; Alpha-1C adrenergic receptor; Alpha-adrenergic receptor 1c
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
Gene References into Functions
  1. This study provided proof that SNPs within ADRA1 is significantly associated with the susceptibility to and the prognosis of cervical vertigo, so that NPY and NA might serve as the precise diagnostic marker and treatment target of cervical vertigo. PMID: 29197114
  2. Our novel data, describing the alpha1A-AR phosphorylation sites, suggest that the observed different phosphorylation patterns may participate in defining adrenoceptor localization and action, under the different conditions examined PMID: 28888989
  3. The presence of ADRA1A autoantibodies is an independent predictor for aortic stiffness progression in normotensive subjects. PMID: 29097625
  4. A mutation of rs2322333, located in the intronic and 3'UTR part of the gene ADRA1A in chromosome 8, has a probability of being deleterious the abolition of ELAV-like protein 1 (ELAVL1) binding site rs2695260. SNP rs2322333 in adrenergic receptor alpha1 (ADRA1A) was higher in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis compared with healthy controls PMID: 27835969
  5. Demonstrate the utility of alpha1 -AR agonists in the treatment of traction alopecia and hair shedding during cosmetic procedures. PMID: 26678522
  6. Immunoreactivity for ADRA1A was densely distributed in vascular smooth muscle of nasal turbinates. PMID: 26739946
  7. alpha1A-adrenoceptor subtypes predominantly contributed to human IAS contraction PMID: 25913761
  8. Data are presented that indicate that Iso represents a novel type of alpha1A-AR partial agonist with signaling bias toward MAPK/ERK signaling cascade that is likely independent of coupling to Galphaq. PMID: 25606852
  9. Data suggested ADRA1A Arg347Cys polymorphism as susceptibility factor in patients with vasovagal syncope; ArgArg genotype could be a marker for vasovagal syncope susceptibility in the Mexican population PMID: 24548768
  10. The ADRA1A mutant triggers two distinct EGFR transactivation-dependent signaling pathways: 1) constitutive Gq-independent beta-arrestin-1/Src/MMP/EGFR/ERK-dependent hyperproliferation and 2) agonist-induced Gq- and EGFR/STAT-dependent hypertrophy. PMID: 24835978
  11. The alpha-AR-induced mydriasis in mice is mediated mainly by the alphaA-AR, with a smaller contribution of the alphaB-AR, matching the relative abundance of these subtypes at the mRNA level. PMID: 25425303
  12. alpha1-AR expression is greater on nerve fibers in skin affected by complex regional pain syndrome type II (CRPS) than in unaffected skin from the same patient or from pain-free controls. PMID: 24342464
  13. Adrenergic alpha1A receptor subtypes transactivate epidermal growth factor receptors. PMID: 23902938
  14. alpha1A-AR, when located in the plasma membrane, signal through calcium and ERK1/2 pathways but, when it translocates to deeper endosomes, continues signaling through ERK1/2 and could also activate the p38 pathway. PMID: 23717684
  15. Upon receptor activation, prostate alpha1A-adrenoceptors bind clathrin LCA. PMID: 23460120
  16. Presence of the Arg347 allele in the ADRA1A gene is a risk factor for occurrence of more severe metabolic abnormalities in patients with schizophrenia. PMID: 22037178
  17. Arg492Cys polymorphism in the alpha(1A) -AR gene was associated with heart rate reactivity to stress in young African Americans. PMID: 22091949
  18. [review] Altered adrenergic receptor signaling following brain trauma contributes to memory dysfunction. PMID: 21792097
  19. Data show that elevated levels of matrix metalloproteinase-7 (MMP7) and a disintegrin and metalloproteinase-12 (ADAM12) in alpha(1a)-247R-expressing cells are responsible for EGF receptor (EGFR) transactivation. PMID: 22089237
  20. Alpha1-adrenoceptor signaling in the human prostate involves regulation of p38 mitogen-activated protein kinase PMID: 21982020
  21. Data suggest that the endocytic pathway is involved in alpha(1A)-AR-induced ERK1/2 activation, which is independent of G(q)/PLC/PKC signaling. PMID: 21738688
  22. Study of alpha1-AR subtype expression on both human monocytes and macrophages illustrates a mechanism by which increased interleukin (IL)-1beta production can be modulated by alpha1-AR input. PMID: 21571945
  23. The mRNA expression of alpha1a-AR subtypes in bladder detrusor and posterior urethra was significantly higher in the inflammation group than in controls. PMID: 21223784
  24. the rs17055869 SNP near the 3' end of ADRA1A is significantly associated with metabolic syndrome and it may be involved in determining a greater level of sympathetic activation in metabolic syndrome patients PMID: 21519279
  25. interactions between metal ions and the alpha(1A)-adrenoceptor with affinities compatible with physiological concentrations PMID: 21262225
  26. Genetic variations in alpha-1A and beta-2 adrenergic receptors (ADRA1A, ADRB2) have been associated with changes in smooth muscle tone in various tissues, and implicated in bronchial hyper-responsiveness, metabolic syndrome, and other disorders. PMID: 19730237
  27. No association of the polmorphisms Arg492Cys (ADRA1A gene), Ser49Gly and Arg389Gly (ADRB1), Arg16Gly and Gln27Glu (ADRB2), 825C/T (GNB3), -1021C/T (DBH) and S/L (SLC6A4) with both tilt test outcomes and new syncopal episodes was found PMID: 20129829
  28. This study suggests the rs1048101 single nucleotide polymorphism within the alpha1a-adrenoceptor as one risk factor for the development of CRPS I after the distal radius fracture. PMID: 20173430
  29. Report a new alpha(1A)AR-CMC-online-HPLC/MS method can be applied for screening active components acting on alpha(1A)AR from traditional Chinese medicines exemplified by Radix Caulophylli. PMID: 20004544
  30. Our study suggests that the ADRA1A gene is involved in weight gain among schizophrenia patients treated with antipsychotics. PMID: 19918262
  31. alpha(1A)-adrenoceptor stimulation in androgen-independent prostate cancer cells via caveolae constitutes one of the mechanisms contributing to their protection from thapsigargin-induced apoptosis PMID: 19763272
  32. the inheritance of polymorphisms in the ADRA2A and ADRA1C genes in 113 nuclear families provided no significant evidence for linkage for these two genes; these genes are not major genetic factors contributing to the susceptibility to GTS PMID: 12707939
  33. alpha1-ARs play a role in promoting human prostate cancer epithelial cell proliferation via transient receptor potential channels PMID: 12782672
  34. Candidate gene for benign prostatic hyperplasia. PMID: 15136785
  35. alpha1A-adrenoceptor structural computer model PMID: 15474515
  36. alpha(1a)-ARs recycle rapidly by an agonist-independent, constitutive, beta-arrestin-dependent process and that this can transport "alpha-blockers" into cells carrying these receptors. PMID: 15626751
  37. The N terminus of RGS2 was required for association with alpha1A-ARs and inhibition of signaling, and amino acids Lys219, Ser220, and Arg238 within the alpha1A-AR i3 loop were found to be essential for this interaction PMID: 15917235
  38. Genetic variations in the ADRA1A gene are significantly associated with essential hypertension in this Chinese population. PMID: 16685204
  39. mTLD interacts with alpha(1A)-AR directly, alters the subcellular localization of receptor, and influences agonist-induced alpha(1A)-AR internalization and calcium signaling. PMID: 16690783
  40. The presence of alpha(1A)-adrenoceptors in epididymal smooth muscle and epithelial cells indicates their contribution to smooth muscle contractile responses and a possible role in the activities of the epithelium lining the epididymal duct. PMID: 18351393
  41. These findings suggest that stimulation of cutaneous alpha(1)-adrenoceptors increased the excitability of heat-sensitized nociceptive afferents. PMID: 18524654
  42. These data provide evidence for a potential clinical relevance for alpha(1)-AAB in hypertensive patients, and the notion of immunity as a possible cause of hypertension. PMID: 19011682
  43. Arg347Cys polymorphism is associated with blood pressure phenotypes, in a large sample of Brazilians from an urban population PMID: 19105822
  44. Administration of an alpha-1A-specific blocker reduced analgesic dosage and colic episodes after shock wave lithotripsy of lower ureteral stones. PMID: 19322003
  45. strongest signal emerged from SNPs in the promoter region (rs3808585) and in an intron (rs17426222, rs4732682, rs573514) of ADRA1A, all located within the same haplotype block PMID: 19352218
  46. Arg347Cys polymorphism of alpha1A-adrenoceptor gene is associated with blood pressure response to nifedipine GITS. PMID: 19444285
  47. The localization of alpha1AAR in lipid rafts directly impacts both basal and activated receptor signaling. PMID: 19520158
  48. Adrenergic receptor (alpha(1A)-AR, beta(2)-AR, and beta(3)-AR) gene polymorphisms are related to the risk of developing fibromyalgia (FM) and are also linked to different domains of the FM syndrome. PMID: 19565482

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Subcellular Location Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasm. Membrane, caveola. Note=Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
Protein Families G-protein coupled receptor 1 family, Adrenergic receptor subfamily, ADRA1A sub-subfamily
Tissue Specificity Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with hi
Database Links

HGNC: 277

OMIM: 104221

KEGG: hsa:148

STRING: 9606.ENSP00000369960

UniGene: Hs.52931

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