Very nice to receive your inquiry.
1. As the following thread suggests a method of phosphomimic mutant, this method is only sutibale to S and T. The Y doesn't have a structurally similar amino acid that can be used as an alternative after phosphorylation.
In addition, Phos-tag SDS-PAGE is just a detection technique, as it's described in the link: " I attach our article, which demonstrated in vitro phosphorylation of recombinant proteins by using Phos-tag SDS-PAGE"
They use a word of demonstrate, but this article mainly introduce three methods to distinguish non phosphorylated and phosphorylated proteins.
The first method is to detect the activity, and the second method is the time course changes of phosphorylation levels, signal pathway is a common used method.The third method is indirect detection by using of inhibitor. the whole article doesn't mention the in vitro phosphorylation of recombinant proteins by using Phos-tag SDS-PAGE
2. If you just want to introduce a phosphorylation at the site of Y23, then the peptide synthesis is a simple method. If you need we can provide further information.
3.If you want a phosphorylation at the site of Y23, and doesn't concern if the other sites will also be phosphorylated.
You can order eukaryotic system expressed protein except for E.coli expression system, because this website http://www.uniprot.org/uniprot/P07355 introduce the post translational modification of this protein.
There are 4 acetylation sites and 4 phosphorylation sites, of which 4 phosphorylation sites, including the site of 24th Y, which refers to the Y23.
So this protein which is expressed by expression host with the ability of post translational modification is likely to be phosphorylated at the Y23 site.
Recombinant Human Annexin A2(ANXA2)
CSB-YP001840HU 30-40 working days
CSB-BP001840HU 30-50 working days
CSB-MP001840HU 30-40 working days
Expression Region :1-339aa; Full length.
Tag information:All provide the N-terminal His-tag.
4.In addition, based on the third project, if your customer don't want the other 4 acetylation sites or 3 phosphorylation sites,
We can conduct mutation, to mutant with structurally similar amino acid that can have very a little influence on the protein structure.
So this protein is phosphorylated only at the Y23 site.