Code | CSB-YP001840HU |
Size | Pls inquire |
Source | Yeast |
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Code | CSB-EP001840HU-B |
Size | Pls inquire |
Source | E.coli |
Conjugate | Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. |
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Code | CSB-BP001840HU |
Size | Pls inquire |
Source | Baculovirus |
Have Questions? | Leave a Message or Start an on-line Chat |
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I would like to find a source of Human Tyr 23 Phosphorylated Annexin A2.
Is there a way to make this recombinant protein so that it is phosphorylated at Tyr 23?
I was quite interested to find the following thread which suggests a couple of approaches to a similar problem involving phosphomimic mutants or alternatively the procedure in the attached article for in vitro phosphorylation of recombinant proteins by using Phos-tag SDS-PAGE.
https://www.researchgate.net/post/How_to_perform_an_in_vitro_phosphorylation_of_recombinant_protein
MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD