Recombinant Human Annexin A2(ANXA2)

Code CSB-YP001840HU
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Source Yeast
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Code CSB-EP001840HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001840HU
Size Pls inquire
Source Baculovirus
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Product Details

Purity >85% (SDS-PAGE)
Target Names ANXA2
Uniprot No. P07355
Alternative Names Annexin A2; Annexin II; Annexin II; heavy chain; Annexin-2; ANX 2; ANX2; ANX2L4; ANXA2; ANXA2_HUMAN; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide (p36); Calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8 ; Chromobindin-8; Epididymis secretory protein Li 270; HEL S 270; LIP2; Lipocortin II; LPC2; LPC2D; p36; P36 protein ; PAP-IV; Placental anticoagulant protein IV; Protein I
Species Homo sapiens (Human)
Expression Region 2-339
Target Protein Sequence STVHEILCK LSLEGDHSTP PSAYGSVKAY TNFDAERDAL NIETAIKTKG VDEVTIVNIL TNRSNAQRQD IAFAYQRRTK KELASALKSA LSGHLETVIL GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDII SDTSGDFRKL MVALAKGRRA EDGSVIDYEL IDQDARDLYD AGVKRKGTDV PKWISIMTER SVPHLQKVFD RYKSYSPYDM LESIRKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM LKIRSEFKRK YGKSLYYYIQ QDTKGDYQKA LLYLCGGDD
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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 Q&A
Q:

I would like to find a source of Human Tyr 23 Phosphorylated Annexin A2.
Is there a way to make this recombinant protein so that it is phosphorylated at Tyr 23?
I was quite interested to find the following thread which suggests a couple of approaches to a similar problem involving phosphomimic mutants or alternatively the procedure in the attached article for in vitro phosphorylation of recombinant proteins by using Phos-tag SDS-PAGE.
https://www.researchgate.net/post/How_to_perform_an_in_vitro_phosphorylation_of_recombinant_protein

A:
Very nice to receive your inquiry.
1. As the following thread suggests a method of phosphomimic mutant, this method is only sutibale to S and T. The Y doesn't have a structurally similar amino acid that can be used as an alternative after phosphorylation.
In addition, Phos-tag SDS-PAGE is just a detection technique, as it's described in the link: " I attach our article, which demonstrated in vitro phosphorylation of recombinant proteins by using Phos-tag SDS-PAGE"
They use a word of demonstrate, but this article mainly introduce three methods to distinguish non phosphorylated and phosphorylated proteins.
The first method is to detect the activity, and the second method is the time course changes of phosphorylation levels, signal pathway is a common used method.The third method is indirect detection by using of inhibitor. the whole article doesn't mention the in vitro phosphorylation of recombinant proteins by using Phos-tag SDS-PAGE
2. If you just want to introduce a phosphorylation at the site of Y23, then the peptide synthesis is a simple method. If you need we can provide further information.
3.If you want a phosphorylation at the site of Y23, and doesn't concern if the other sites will also be phosphorylated.
You can order eukaryotic system expressed protein except for E.coli expression system, because this website http://www.uniprot.org/uniprot/P07355 introduce the post translational modification of this protein.
There are 4 acetylation sites and 4 phosphorylation sites, of which 4 phosphorylation sites, including the site of 24th Y, which refers to the Y23.
So this protein which is expressed by expression host with the ability of post translational modification is likely to be phosphorylated at the Y23 site.
Recombinant Human Annexin A2(ANXA2)
CSB-YP001840HU 30-40 working days
CSB-BP001840HU 30-50 working days
CSB-MP001840HU 30-40 working days
Expression Region :1-339aa; Full length.
Tag information:All provide the N-terminal His-tag.
Sequence:

MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD


4.In addition, based on the third project, if your customer don't want the other 4 acetylation sites or 3 phosphorylation sites,
We can conduct mutation, to mutant with structurally similar amino acid that can have very a little influence on the protein structure.
So this protein is phosphorylated only at the Y23 site.

Target Background

Function
(From Uniprot)
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9
Gene References into Functions
  1. Mechanistic studies revealed that FOXD2AS1 upregulated the expression of the miR206 target gene ANXA2 in hepatocellular carcinoma by acting as a miR206 sponge. PMID: 30272362
  2. study has localised AnxA2/S100A10 complexes to key anatomical locations in the placenta and suggests a role for this complex in amniotic epithelium, trophoblasts and syncytium, in addition to its well-known roles in endothelial cells PMID: 30143909
  3. Inhibition of ANXA2 will affect the expression of downstream targets and downstream signaling pathways, leading to the suppression of the malignant progression of tumors. PMID: 30355917
  4. our data suggest that overexpression of peroxiredoxin-2, annexin A2, and heat shock protein beta-1 was correlated with tumor invasion, metastasis, and poor prognosis, and therefore, these proteins may serve as potential diagnostic and therapeutic biomarkers. PMID: 29332450
  5. aptamer wh6 can block MM cell adhesion to ANXA2 and block the proliferation of MM cells induced by ANXA2 PMID: 29906496
  6. The results demonstrated that ANX2 and stromal tenascin C regulated invasion in addition to stemness and anoikis resistance, which are crucial for metastasis in the progression of pancreatic ductal adenocarcinoma. PMID: 29749431
  7. highly expressed in gastric cancer, related to tumor size, histological differentiation, lymph node metastasis PMID: 29097873
  8. models for annexin A2-mediated vesicle aggregation mechanisms. PMID: 29567212
  9. Our profiling data provided a rich source for further study of the molecular mechanisms of acquired drug resistance inneuroblastoma ( NB). Further study may determine the role of ANXA2 as a prognostic biomarker and a potential therapeutic target for patients with multidrug-resistant NB. PMID: 28814318
  10. These results suggested that increased ANXA2 and less expressed beta-catenin were correlated to adenomyosis-associated dysmenorrhea PMID: 28547742
  11. These data suggested that Annexin A2 induces cisplatin resistance of non-small cell lung cancer (NSCLC)via regulation of JNK/c-Jun/p53 signaling, and provided an evidence that blockade of Annexin A2 could serve as a novel therapeutic approach for overcoming drug resistance in NSCLCs PMID: 28886730
  12. Results show that Anxa2-Tyr23 phosphorylation is necessary for proliferation, invasion, and metastasis of breast cancer cells in vitro and in vivo. Tyr23-phosphorylated Anxa2 binds to STAT3 and serves as a key regulator of STAT3 signaling. PMID: 28470457
  13. annexin A2 can potentially predict the development of pre-eclampsia. The low annexin A2 levels (<0.89 ng/ml) together with higher blood pressure and proteinuria constitute a higher risk to develop preeclampsia. PMID: 28501283
  14. Variants in the Annexin A2 (ANXA2) gene directly influence plasma LDL-C levels. PMID: 28456096
  15. this study showed that Annexin A2 inhibition suppresses proliferation and invasion in ovarian cancer via beta-catenin/EMT, proposing the potential role of Annexin A2 in the prevention and treatment of ovarian cancer PMID: 28440436
  16. FNDC3B and ANXA2 expression correlate negatively with patient survival in hepatocellular carcinoma PMID: 27385217
  17. UBAP2 formed a complex with Annexin A2 and promoted the degradation of Annexin A2 protein by ubiquitination, and then inhibited HCC progression. PMID: 27121050
  18. results indicate that ANXA2 plays an important role to enhance the malignant behaviors of Hepatocellular carcinoma cells, and the enhancement is closely based on its remodeling to cell structures PMID: 27060670
  19. Thus, tight regulation of AnxA2 function is an integral aspect of cellular homeostasis. The presence of AnxA2 in cancer cell-derived exosomes, as well as the potential regulation of exosomal AnxA2 by phosphorylation or other PTMs, are topics of great interes PMID: 28867585
  20. Restoration of miR-101 expression reversed chemoresistance of drug-resistant gastric cancer cells by inhibiting viability and enhancing apoptosis via targeting ANXA2. PMID: 28609840
  21. Annexin 2A (ANXA2) is over-expressed in Glioblastoma (GBM) and positively correlates with patient outcome. PMID: 27429043
  22. Results confirm that both circRNA expression and even ANXA2 expression could be used as biomarkers of RR-MS with good values of specificity and sensitivity. PMID: 28651352
  23. This study demonstrated that the expression level of AnxA2 was significantly higher in recurrent adamantinomatous craniopharyngioma compared with primary ones. AND AnxA2(+) adamantinomatous craniopharyngioma cells exhibited enhanced proliferation and migration ability compared with AnxA2(-) adamantinomatous craniopharyngioma cells. PMID: 27640198
  24. Cercvical cancer stem cells express annexin II, an HPV co-receptor. PMID: 27008711
  25. Annexin A2 (ANXA2) and prostate-specific antigen (PSA) proteins measured from biopsy tumour regions are unlikely to be good biomarkers for prediction of the clinical outcome of prostate cancer presenting with apparently localized disease. PMID: 29187477
  26. anti-ANXA2 antibodies play a role in thrombotic mechanisms leading to recurrent pregnancy loss and placental vascular disease PMID: 27631133
  27. results suggest that mycoplasma induces a resistance to multiple drugs in hepatocarcinoma cells which required the interaction of P37 and Annexin A2. The pathway downstream this interaction needs to be explored PMID: 28976984
  28. Findings indicate that Munc13-4 supports acute WPB exocytosis by tethering WPBs to the plasma membrane via AnxA2-S100A10. PMID: 28450451
  29. Detecting ANXA2 and ANXA4 expression may aid the evaluation of cervical carcinoma prognosis. PMID: 27402115
  30. our studies show that this G-motif represents a novel and essential determinant for axonal localization of the Anxa2 mRNA mediated by the SMN complex. PMID: 28258160
  31. The data data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. PMID: 27863502
  32. Exosome-associated Annexin II plays an important role in angiogenesis and breast cancer metastasis. PMID: 27760843
  33. ANXA2 stromal expression might play a key role in aggressive tumor phenotype associated with increased EMT CTCs release, however, other factors beyond ANXA2 are responsible for coagulation activation mediated by CTCs in breast cancer patients. PMID: 28476852
  34. annexin A2 contributes to lung injury and fibrotic disease by mediating the fibrogenic actions of FXa. PMID: 28283478
  35. Annexin A2 and HB-EGF are overexpressed and are being secreted into serum in Her-2 negative breast cancer patients. PMID: 27496793
  36. results indicate that ANXA2 facilitates PS-ASO trafficking from early to late endosomes where it may also contribute to PS-ASO release PMID: 27378781
  37. Annexins A2 and A8 in endothelial cell exocytosis and the control of vascular homeostasis PMID: 27451994
  38. Rack1 regulated P-Glycoprotein activity, which was necessary for adriamycin-induced P-Glycoprotein - mediated phosphorylation of Anxa2 and Erk1/2. PMID: 27754360
  39. The presence of vascular lesions in lupus nephritis (LN) appears to be associated with significant differences in the vascular expression of ANXA2. PMID: 26511441
  40. expression elevated in lung squamous cell carcinoma tissue PMID: 28178129
  41. annexin A2 but not follistatin is expressed in hepatocellular carcinoma PMID: 26189841
  42. Our results place ANXA2 at the apex of a regulatory cascade that determines glioblastoma mesenchymal transformation and validate aSICS as a general methodology to uncover regulators of cancer subtypes. PMID: 27667176
  43. Overexpression of ANXA2 is associated with colorectal cancer. PMID: 27468721
  44. Annexin A2, up-regulated by IL-6, promotes ligament ossification in ankylosing spondylitis patients. PMID: 27697640
  45. highly specific 30 kDa plant lectin could dissociate AnxA2 from endogenous lectin galectin-3 interaction at the cell surface. This dissociation could down-regulate Bcl-2 family proteins, cell proliferation, and migration simultaneously triggering cell apoptosis PMID: 26438086
  46. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
  47. annexin A2 and S100A10 expressions are powerful predictors of serous ovarian cancer outcome. PMID: 26925708
  48. expression of ANXA2 is associated with tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma PMID: 26196246
  49. Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer. PMID: 26307676
  50. the role of Axna2 is instructive for understanding the development of RA, suppress the effect of Axna2 might provide a new potential measure for treatment of RA. PMID: 26963384

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Subcellular Location Secreted, extracellular space, extracellular matrix, basement membrane, Melanosome
Protein Families Annexin family
Database Links

HGNC: 537

OMIM: 151740

KEGG: hsa:302

STRING: 9606.ENSP00000346032

UniGene: Hs.511605

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