Recombinant Human Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (ARAP1), partial

Code CSB-YP856998HU
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Source Yeast
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Code CSB-EP856998HU
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Source E.coli
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Code CSB-EP856998HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856998HU
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Source Baculovirus
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Code CSB-MP856998HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARAP1
Uniprot No.
Alternative Names
2410002L19Rik; ANK repeat and PH domain-containing protein 1; ARAP 1; Arap1; ARAP1_HUMAN; Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domains containing protein 1; Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domain containing protein 1; Arf-GAP with Rho-GAP domain; CENT D2; Centaurin delta 2; Centaurin delta2; Centaurin-delta-2; CENTD 2; CENTD2; Cnt d2; Cnt-d2; Cntd2; KIAA0782; MGC176140; mKIAA0782
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
Gene References into Functions
  1. Quantitative trait loci genes analysis reveals association between T2D risk variants at the ARAP1/STARD10 locus and STARD10 but not ARAP1 expression. PMID: 28132686
  2. Genetic variant rs1552224 of CENTD2 on chromosome 11 contributes to an independent effect as well as joint cumulative effect with rs2237897 of KCNQ1 on the risk of type 2 diabetes mellitus in Chinese Han population. PMID: 25749274
  3. The proinsulin-decreasing and T2D-risk Alleles are associated with increased ARAP1 mRNA levels in primary human pancreatic islets. PMID: 24439111
  4. a novel molecular mechanism of circular dorsal ruffles ring size control through the ARAP1-Arf1/5 pathway. PMID: 22573888
  5. Arap1 is highly expressed in the renal vasculature, and its expression is suppressed by angiotensin II. PMID: 22357923
  6. Of the lead variants from 12 novel type 2 diabetes associated loci, CENTD2 significantly associated with increased plasma glucose values and decreased glucose-stimulated insulin release. PMID: 21267535
  7. ARAP1 associated with CIN85 affects epidermal growth factor receptor endocytic trafficking PMID: 21275903
  8. PTK6 enhances EGFR signaling by inhibition of EGFR down-regulation through phosphorylation of ARAP1 in breast cancer cells. PMID: 20554524
  9. ARAP1 acts primarily on Arf6 in vivo. PMID: 18003747
  10. ARAP1 knockdown significantly compromises the localization of death receptor 4 at the cell surface of several tumor cell lines and slows down their TRAIL-induced death. PMID: 18165900
  11. ARAP1 controls the late steps of the endocytic trafficking of the EGF-R PMID: 18764928
  12. ARAP1 regulates the endocytic traffic of EGFR and, consequently, the rate of EGFR signal attenuation PMID: 18939958
  13. Data suggest that ARAP1 is recruited to membranes independently of PtdIns(3,4,5)P(3), the subsequent production of which triggers enzymatic activity. PMID: 19666464

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Subcellular Location
Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell membrane. Note=Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
Tissue Specificity
Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
Database Links

HGNC: 16925

OMIM: 606646

KEGG: hsa:116985

STRING: 9606.ENSP00000377233

UniGene: Hs.503165

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