Recombinant Human Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1(ASAP1) ,partial

Code CSB-YP891546HU
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Source Yeast
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Code CSB-EP891546HU
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Source E.coli
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Code CSB-EP891546HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891546HU
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Source Baculovirus
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Code CSB-MP891546HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ASAP1
Uniprot No. Q9ULH1
Alternative Names 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein; 130 kDa phosphatidylinositol 4; 5-biphosphate-dependent ARF1 GTPase-activating protein; ADP ribosylation factor directed GTPase activating protein 1; ADP-ribosylation factor-directed GTPase-activating protein 1; AMAP 1; AMAP1; ANK repeat and PH domain-containing protein 1; ARF GTPase activating protein 1; ARF GTPase-activating protein 1; Arf-GAP with SH3 domain; ASAP 1; ASAP1; ASAP1_HUMAN; Centaurin beta 4; CentB4; DDEF 1; DDEF1 protein; DEF-1; Development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; KIAA1249; PAG 2; PAG2; PAP; PIP2 dependent ARF1 GAP; PIP2-dependent ARF1 GAP; ZG 14P; ZG14P
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.
Gene References into Functions
  1. AMAP1 mediated CCL18-induce activation of NF-kappaB and promoted breast cancer metastasis. PMID: 28834540
  2. Results indicate that monocyte differentiation involves enhancement of IRES activity, by which protein levels of AMAP1 are primarily upregulated. PMID: 18843202
  3. Less differentiated embryonal and undifferentiated small hepatoblastoma cells progressively lose EGFR and ASAP1 expression. This trend is exaggerated in unresectable, locally invasive or metastatic tumors. PMID: 27910913
  4. eIF5B promoted hepatocellular carcinoma cell proliferation and migration in vitro and in vivo partly through increasing ASAP1 expression. PMID: 27694689
  5. results, specifically, the absence of associations of polymorphisms of ASAP1 with tuberculosis (TB) risk, suggest that ASAP1 may not be a susceptibility gene for TB in Western Chinese Han and Tibetan population PMID: 27227929
  6. results support the hypothesis that ASAP1 is a positive regulator of NM2A. PMID: 26893376
  7. Rab11-FIP3 coordinates the interactions of ASAP1 and Rab11a with Rabin8. PMID: 25673879
  8. The activation of NF-kappaB induced translocation of AMAP1 to cytoplasm from cell membrane and nucleus, which resulted in augmented interaction of AMAP1 and IKKbeta PMID: 24865276
  9. High ASAP1 expression, that was reduced after Mycobacterium tuberculosis infection, and rs10956514, are associated with the level of reduction of ASAP1 expression. PMID: 25774636
  10. ASAP1 appears to contribute to the malignant mechanism of LSCC and may represent a significant prognostic marker for laryngeal squamous cell carcinoma patients. PMID: 24788532
  11. results indicated that elevated expression of ASAP1 plays an important role in the progression and metastasis of ovarian cancer PMID: 24427349
  12. Data suggest that GEP100-Arf6-AMAP1-cortactin pathway, activated by VEGFR2, appears to be common in angiogenesis and cancer invasion and metastasis, and provides their new therapeutic targets. PMID: 21858086
  13. GEFH1 was identified as binding partner for the BAR domain of ASAP1.GEFH1 is a negative regulator of podosomes. PMID: 21352810
  14. ASAP1 can contribute to the dissemination of a variety of tumor types and represent a potential target for cancer therapy. PMID: 20154719
  15. DEF-1 alters cell motility through the deactivation of ARF1. In contrast, the inhibition of cell spreading by DEF-1 was not dependent on GAP activity, indicating that spreading and motility are altered by DEF-1 through different pathways. PMID: 11773070
  16. These results suggest that POB1 interacts with PAG2 through its proline-rich motif, thereby regulating cell migration (PAG2). PMID: 12149250
  17. involved in peripheral focal adhesions, directed by CRKL protein PMID: 12522101
  18. DDEF1 overexpression may be a pathogenetically relevant consequence of chromosome 8q amplification, which commonly occurs in high-grade uveal melanomas PMID: 15897555
  19. Results support a model that regulation of GAP (GTPase-activating protein) activity of ASAP1 involves conformational changes, coincident with recruitment to a membrane surface and following the specific binding of phosphatidylinositol 4,5-bisphosphate. PMID: 16038802
  20. Results suggest that the AMAP1/cortactin complex, which is not detected in normal mammary epithelial cells, is an excellent drug target for cancer therapeutics. PMID: 16636290
  21. ASAP3 functions nonredundantly with ASAP1 to control cell movement and may have a role in cancer cell invasion. PMID: 18400762
  22. study suggests that the ASAP1 gene plays a role in prostate cancer metastasis and may represent a therapeutic target and/or biomarker for metastatic disease PMID: 18519696
  23. ASAP1, like FIP3, functions as a component of the endocytic recycling compartment PMID: 18685082
  24. Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1 PMID: 19017632

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Subcellular Location Cytoplasm, Membrane
Database Links

HGNC: 2720

OMIM: 605953

KEGG: hsa:50807

STRING: 9606.ENSP00000350297

UniGene: Hs.655552

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