Recombinant Human BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1)

Code CSB-YP622686HU
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Source Yeast
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Code CSB-EP622686HU
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Source E.coli
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Code CSB-EP622686HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622686HU
MSDS
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Source Baculovirus
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Code CSB-MP622686HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TNFAIP1
Uniprot No.
Alternative Names
TNFAIP1; BACURD2; EDP1BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2; hBACURD2; BTB/POZ domain-containing protein TNFAIP1; Protein B12; Tumor necrosis factor; alpha-induced protein 1; endothelial
Species
Homo sapiens (Human)
Expression Region
1-316
Target Protein Sequence
MSGDTCLCPA SGAKPKLSGF KGGGLGNKYV QLNVGGSLYY TTVRALTRHD TMLKAMFSGR MEVLTDKEGW ILIDRCGKHF GTILNYLRDD TITLPQNRQE IKELMAEAKY YLIQGLVNMC QSALQDKKDS YQPVCNIPII TSLKEEERLI ESSTKPVVKL LYNRSNNKYS YTSNSDDHLL KNIELFDKLS LRFNGRVLFI KDVIGDEICC WSFYGQGRKL AEVCCTSIVY ATEKKQTKVE FPEARIYEET LNVLLYETPR VPDNSLLEAT SRSRSQASPS EDEETFELRD RVRRIHVKRY STYDDRQLGH QSTHRD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
Gene References into Functions
  1. TNFAIP1 silence significantly increased the migrated and invaded cells compared to that in control, while these increases were abolished by miR-424 suppression. PMID: 28535539
  2. CREB is a negative regulator of the TNFAIP1 gene. PMID: 26398148
  3. TNFAIP1 plays an important role in mediating miR-15a dependent biological functions in osteosarcoma. PMID: 26261520
  4. MiR-181a played a critical role in regulating pancreatic cancer growth and migration, likely interacting with TNFAIP1. PMID: 26152285
  5. Results showed that the expression of TNFAIP1 protein was significantly increased in osteosarcoma tissues and associated with distant metastasis. PMID: 24969828
  6. Expression of TNFAIP1 is regulated by the transcriptional factor Sp1. PMID: 23912453
  7. TNFAIP1 inhibited the transcriptional activities of nuclear factor kappa B (NF-kappaB) and activating protein-1 reporters PMID: 22810651
  8. CK2 could phosphorylate TNFAIP1 in vitro and in vivo, which facilitated the distribution of TNFAIP1 in nucleus and enhanced its interaction with PCNA. PMID: 19851886
  9. The promoter region of human TNFAIP1 gene was functionally characterized. PMID: 19593659
  10. suggest that the TNFAIP1/POLDIP2 complex sense-antisense architecture represents a clinically significant transcriptional structural-functional gene module associated with amplification of the genomic region on 17q11.2 in breast cancer. PMID: 20158880
  11. TNFAIP1 shows increased transcript levels in Alzheimer's disease brains PMID: 12600716
  12. Expression levels of TNFAIP1 are high in COS7 and NIH3T3 cell lines but low in the cancer cell lines. PMID: 16870576

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Subcellular Location
Cytoplasm. Nucleus. Endosome. Note=Colocalizes with RHOB in endosomes.
Protein Families
BACURD family
Database Links

HGNC: 11894

OMIM: 191161

KEGG: hsa:7126

STRING: 9606.ENSP00000226225

UniGene: Hs.76090

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