Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

NAIP

Uniprot No.

Q13075

Research Area

Cancer

Alternative Names

Baculoviral IAP repeat containing 1; Baculoviral IAP repeat-containing protein 1; BIRC 1; BIRC1; BIRC1_HUMAN; Birc1a; FLJ42520; NAIP; Naip1; Neuronal apoptosis inhibitory protein; NLR family apoptosis inhibitory protein; NLR family BIR domain containing 1; NLRB 1; NLRB1; Nucleotide binding oligomerization domain leucine rich repeat and BIR domain containing 1; Psi neuronal apoptosis inhibitory protein; psiNAIP; Similar to occludin

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

60-345aa

Target Protein Sequence

EAKRLKTFVTYEPYSSWIPQEMAAAGFYFTGVKSGIQCFCCSLILFGAGLTRLPIEDHKRFHPDCGFLLNKDVGNIAKYDIRVKNLKSRLRGGKMRYQEEEARLASFRNWPFYVQGISPCVLSEAGFVFTGKQDTVQCFSCGGCLGNWEEGDDPWKEHAKWFPKCEFLRSKKSSEEITQYIQSYKGFVDITGEHFVNSWVQRELPMASAYCNDSIFAYEELRLDSFKDWPRESAVGVAALAKAGLFYTGIKDIVQCFSCGGCLEKWQEGDDPLDDHTRCFPNCPFL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

48.6kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human NAIP covers amino acids 60-345. This NAIP protein is theoretically predicted to have a molecular weight of 48.6 kDa. Expression of this NAIP protein is conducted in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the NAIP encoding gene fragment was conducted, allowing for easier detection and purification of the NAIP protein in subsequent stages.

Human baculoviral IAP repeat-containing protein 1 (NAIP) is crucial for innate immunity, acting as a sensor for intracellular pathogens. NAIP, part of the NLRC4 inflammasome, recognizes bacterial flagellin and type III secretion systems, triggering inflammatory responses and antimicrobial defenses. In infectious disease research, studying NAIP provides insights into host-pathogen interactions. Its association with autoinflammatory diseases links NAIP to autoimmune research. Moreover, understanding NAIP contributes to the broader field of inflammasome biology, offering potential therapeutic targets for immune-related disorders. Investigating NAIP's diverse functions provides a comprehensive understanding of its roles in immunity and various pathophysiological conditions.

Target Background

Function

Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.; Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C.violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C.violaceum and L.pneumophila.

Gene References into Functions

Our present report implies that NAIP will have broad implications for ALS symptoms as a risk factor and a promising prognostic biomarker. PMID: 29311650
Data document a previously unknown localization of NAIP along the entire cytokinetic process whose dynamics exhibits a distinct behavior. PMID: 28059125
NAIP expression is most abundant in M2 macrophages, while cIAP1 and cIAP2 show an inverse pattern of expression in polarized cells, cIAP2 is preferentially expressed in M1-macrophages and cIAP1 in M2-macrophages. IAP antagonist treatment of resting M0 macrophages preceding polarization stimulation, induced upregulation of NAIP in M2 and downregulation of cIAP1 in M1 and M2 but an induction of cIAP2 in M1 macrophages. PMID: 29518103
Deletion in NAIP gene is associated with spinal muscular atrophy. PMID: 27754957
NAIP and survivin expressions were significantly reduced following varicocele induction when compared to sham animals whereas PDRN-treated rats showed an increase in NAIP and survivin levels. PMID: 26347229
The copy numbers and gene structures of NAIP genes were different in Chinese spinal muscular atrophy patients and healthy controls PMID: 25888055
results revealed that SMN2 and NAIP copy numbers significantly influenced the age at onset, risk of death, and life expectancy in the spinal muscular atrophy patients and that the effect of SMN2 was more significant PMID: 25330799
human Naip functions to activate the inflammasome in response to flagellin, similar to murine Naip5/6. PMID: 26109648
Modulation of chemotherapeutic drug resistance in neuroblastoma SK-N-AS cells by the neural apoptosis inhibitory protein and miR-520f. PMID: 25137037
Copy number variations of SMN2 and NAIP genes in patients are related to spinal muscular atrophy clinical types (P < 0.05). PMID: 24711022
/NAIP1 and NAIP2/5 formed a large oligomeric complex with NLRC4 in the presence of corresponding bacterial ligands, and could support reconstitution of the NLRC4 inflammasome in a ligand-specific manner. PMID: 23940371
identified an intronic region of the NAIP gene responding to TEAD1/YAP activity, suggesting that regulation of NAIP by TEAD1/YAP is at the transcriptional level PMID: 23994529
the NAIP5-NLRC4 inflammasome is induced by direct interactions with conserved N- and C-terminal regions of flagellin PMID: 23012363
NAIPFull gene duplication might have been evolutionary maintained, or even selected for, because it may confer an advantage to the host against flagellated bacteria PMID: 22067212
There is a close relationship between SMN2, NAIP and H4F5 gene copy number and spinal muscular atrophy disease severity PMID: 21821450
NOD domain is essential for effective inhibition of procaspase-9 and procaspase-3 cleavage by the NAIP protein in apoptosis. PMID: 21371431
an inhibitor of procaspase-9 preventing apoptosis at the initiation stage PMID: 20171302
Expression of NAIP may be associated with enhanced survival of prostate cancer in response to castration PMID: 20044205
Results provide the first structures of BIR domains from human NAIP and cIAP2. PMID: 19923725
NAIP gene deletion was higher in type I spinal muscular atrophy than in type U or V. In type I patients lacking the NAIP gene, deterioration in their respiratory function is more rapid than in those type I patients retaining the NAIP gene. PMID: 11912351
NAIP-deltaEx10-11: a novel splice variant of the apoptosis inhibitor NAIP is differently expressed in drug-sensitive and multidrug-resistant HL60 leukemia cells. NAIP transcripts might be involved in tumor resistance to chemotherapeutic agents. PMID: 12127562
NAIP:Structural requirements for binding hippocalcin and effects on survival of sympathetic neurons. PMID: 12445469
NAIP does not interact with Smac and requires ATP to bind caspase-9 PMID: 15280366
Alterations in C/CAAT enhancer binding protein alpha and neuronal apoptosis inhibitory protein expression occurred in human adipose stromal-vascular cells after weight loss PMID: 15340105
Multiple, domesticated long terminal repeats (LTRs) of endogenous retroviral elements provide NAIP promoter function in human, mouse, and rat. PMID: 17222062
a role for NAIP in increasing the survival of cells undergoing terminal differentiation as well as the possibility that the protein serves as an intestinal pathogen recognition protein was suggested PMID: 17510375
80% neuronal apoptosis inhibitory protein gene deletion in 5q-spinal muscular atrophy patients (91% spinal muscular atrophy-I, 50% spinal muscular atrophy-II and -III), and in 5% (two of forty) of spinal muscular atrophy parents, was found. PMID: 17903057
While there was no evidence of NAIP expression in the normal breast tissue, NAIP was expressed in all breast cancer samples. PMID: 17923748
May be a modifying factor for disease severity of spinal muscular atrophy. PMID: 17932457
The present study is the first one giving detailed information on SMN and NAIP deletion rates in Iranian SMA patients. PMID: 18071605
Data show elevated expression of NAIP in peripheral mononuclear cells from children with Fabry disease. PMID: 18339188
hNAIP and hIpaf mediate innate intracellular defense against flagellated Legionella in human cells. PMID: 18453601
The presence of one NAIP copy, that is, heterozygous NAIP deletion, was common in Vietnamese SMA, regardless of clinical phenotype. PMID: 18533950
HIAP-1 and HIAP-2 mRNA levels were elevated in resting T cells while NAIP mRNA was increased in whole blood in multiple sclerosis PMID: 18566024
in glioma & glioblastoma multiforme, selective upregulation of miRNA-221 & down-regulation of a miRNA-221 mRNA target encoding BIRC1 were observed; expression of BIRC5 & caspase-3 were found to be significantly up-regulated, particularly in stage IV GBM PMID: 18759060
Data show that NAIP deletion predicts disease severity in spinal muscular atrophy. PMID: 18842367
Among the SMA Type I patients, 43% showed deletions of SMN1 and NAIP. PMID: 18974562
findings of homozygous deletions of exon 7 and/or exon 8 of SMN1 gene confirmed the diagnosis of SMA, and suggested that the deletion of SMN1 exon 7 is a major cause of SMA in southern Chinese children. PMID: 19198020
higher number of SMN2 copies makes the clinical symptoms more benign, and the NAIP gene deletion is associated with a more severe phenotype PMID: 19287802
a novel NAIP isoform derives from intragenic Alu SINE promoters PMID: 19488400

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Tissue Specificity

Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.

Database Links

HGNC: 7634

OMIM: 600355

KEGG: hsa:4671

STRING: 9606.ENSP00000428657

UniGene: Hs.646951

Code	CSB-EP615662HU
Abbreviation	Recombinant Human NAIP protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Baculoviral IAP repeat-containing protein 1 (NAIP), partial

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