Product Details

Uniprot No.

Q13075

Target Names

NAIP

Alternative Names

Baculoviral IAP repeat-containing protein 1 (Neuronal apoptosis inhibitory protein), NAIP, BIRC1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human NAIP

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13E6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NAIP, also known as neuronal apoptosis inhibitory protein or BIRC1, serves as a critical regulator of programmed cell death and innate immune responses. As a member of the inhibitor of apoptosis protein family, NAIP plays essential roles in protecting neurons from apoptotic signals and functions as an intracellular pattern recognition receptor within the inflammasome complex. Its involvement in both cell survival pathways and inflammatory responses makes it a compelling target for researchers investigating cancer biology, neurodegenerative conditions, and immune cell function.

This recombinant monoclonal antibody, clone 13E6, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human NAIP and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The affinity-chromatography purification ensures high purity suitable for sensitive detection methods.

Validation in immunohistochemistry demonstrates reliable performance in paraffin-embedded human placenta tissue, with recommended working dilutions of 1:50 to 1:200. The established staining protocol, utilizing citrate buffer antigen retrieval at pH 6.0 followed by overnight primary antibody incubation, provides a solid foundation for researchers to adapt to their specific tissue types and experimental conditions. Additional compatibility with ELISA expands its utility across different experimental platforms.

For investigators exploring apoptosis regulation, inflammasome biology, or the intersection of cell death pathways with cancer progression, this antibody provides a dependable tool for characterizing NAIP expression patterns in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.; Acts as a sensor component of the NLRC4 inflammasome that specifically recognizes and binds needle protein CprI from pathogenic bacteria C.violaceum. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria such as C.violaceum and L.pneumophila.

Gene References into Functions

Our present report implies that NAIP will have broad implications for ALS symptoms as a risk factor and a promising prognostic biomarker. PMID: 29311650
Data document a previously unknown localization of NAIP along the entire cytokinetic process whose dynamics exhibits a distinct behavior. PMID: 28059125
NAIP expression is most abundant in M2 macrophages, while cIAP1 and cIAP2 show an inverse pattern of expression in polarized cells, cIAP2 is preferentially expressed in M1-macrophages and cIAP1 in M2-macrophages. IAP antagonist treatment of resting M0 macrophages preceding polarization stimulation, induced upregulation of NAIP in M2 and downregulation of cIAP1 in M1 and M2 but an induction of cIAP2 in M1 macrophages. PMID: 29518103
Deletion in NAIP gene is associated with spinal muscular atrophy. PMID: 27754957
NAIP and survivin expressions were significantly reduced following varicocele induction when compared to sham animals whereas PDRN-treated rats showed an increase in NAIP and survivin levels. PMID: 26347229
The copy numbers and gene structures of NAIP genes were different in Chinese spinal muscular atrophy patients and healthy controls PMID: 25888055
results revealed that SMN2 and NAIP copy numbers significantly influenced the age at onset, risk of death, and life expectancy in the spinal muscular atrophy patients and that the effect of SMN2 was more significant PMID: 25330799
human Naip functions to activate the inflammasome in response to flagellin, similar to murine Naip5/6. PMID: 26109648
Modulation of chemotherapeutic drug resistance in neuroblastoma SK-N-AS cells by the neural apoptosis inhibitory protein and miR-520f. PMID: 25137037
Copy number variations of SMN2 and NAIP genes in patients are related to spinal muscular atrophy clinical types (P < 0.05). PMID: 24711022
/NAIP1 and NAIP2/5 formed a large oligomeric complex with NLRC4 in the presence of corresponding bacterial ligands, and could support reconstitution of the NLRC4 inflammasome in a ligand-specific manner. PMID: 23940371
identified an intronic region of the NAIP gene responding to TEAD1/YAP activity, suggesting that regulation of NAIP by TEAD1/YAP is at the transcriptional level PMID: 23994529
the NAIP5-NLRC4 inflammasome is induced by direct interactions with conserved N- and C-terminal regions of flagellin PMID: 23012363
NAIPFull gene duplication might have been evolutionary maintained, or even selected for, because it may confer an advantage to the host against flagellated bacteria PMID: 22067212
There is a close relationship between SMN2, NAIP and H4F5 gene copy number and spinal muscular atrophy disease severity PMID: 21821450
NOD domain is essential for effective inhibition of procaspase-9 and procaspase-3 cleavage by the NAIP protein in apoptosis. PMID: 21371431
an inhibitor of procaspase-9 preventing apoptosis at the initiation stage PMID: 20171302
Expression of NAIP may be associated with enhanced survival of prostate cancer in response to castration PMID: 20044205
Results provide the first structures of BIR domains from human NAIP and cIAP2. PMID: 19923725
NAIP gene deletion was higher in type I spinal muscular atrophy than in type U or V. In type I patients lacking the NAIP gene, deterioration in their respiratory function is more rapid than in those type I patients retaining the NAIP gene. PMID: 11912351
NAIP-deltaEx10-11: a novel splice variant of the apoptosis inhibitor NAIP is differently expressed in drug-sensitive and multidrug-resistant HL60 leukemia cells. NAIP transcripts might be involved in tumor resistance to chemotherapeutic agents. PMID: 12127562
NAIP:Structural requirements for binding hippocalcin and effects on survival of sympathetic neurons. PMID: 12445469
NAIP does not interact with Smac and requires ATP to bind caspase-9 PMID: 15280366
Alterations in C/CAAT enhancer binding protein alpha and neuronal apoptosis inhibitory protein expression occurred in human adipose stromal-vascular cells after weight loss PMID: 15340105
Multiple, domesticated long terminal repeats (LTRs) of endogenous retroviral elements provide NAIP promoter function in human, mouse, and rat. PMID: 17222062
a role for NAIP in increasing the survival of cells undergoing terminal differentiation as well as the possibility that the protein serves as an intestinal pathogen recognition protein was suggested PMID: 17510375
80% neuronal apoptosis inhibitory protein gene deletion in 5q-spinal muscular atrophy patients (91% spinal muscular atrophy-I, 50% spinal muscular atrophy-II and -III), and in 5% (two of forty) of spinal muscular atrophy parents, was found. PMID: 17903057
While there was no evidence of NAIP expression in the normal breast tissue, NAIP was expressed in all breast cancer samples. PMID: 17923748
May be a modifying factor for disease severity of spinal muscular atrophy. PMID: 17932457
The present study is the first one giving detailed information on SMN and NAIP deletion rates in Iranian SMA patients. PMID: 18071605
Data show elevated expression of NAIP in peripheral mononuclear cells from children with Fabry disease. PMID: 18339188
hNAIP and hIpaf mediate innate intracellular defense against flagellated Legionella in human cells. PMID: 18453601
The presence of one NAIP copy, that is, heterozygous NAIP deletion, was common in Vietnamese SMA, regardless of clinical phenotype. PMID: 18533950
HIAP-1 and HIAP-2 mRNA levels were elevated in resting T cells while NAIP mRNA was increased in whole blood in multiple sclerosis PMID: 18566024
in glioma & glioblastoma multiforme, selective upregulation of miRNA-221 & down-regulation of a miRNA-221 mRNA target encoding BIRC1 were observed; expression of BIRC5 & caspase-3 were found to be significantly up-regulated, particularly in stage IV GBM PMID: 18759060
Data show that NAIP deletion predicts disease severity in spinal muscular atrophy. PMID: 18842367
Among the SMA Type I patients, 43% showed deletions of SMN1 and NAIP. PMID: 18974562
findings of homozygous deletions of exon 7 and/or exon 8 of SMN1 gene confirmed the diagnosis of SMA, and suggested that the deletion of SMN1 exon 7 is a major cause of SMA in southern Chinese children. PMID: 19198020
higher number of SMN2 copies makes the clinical symptoms more benign, and the NAIP gene deletion is associated with a more severe phenotype PMID: 19287802
a novel NAIP isoform derives from intragenic Alu SINE promoters PMID: 19488400

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Tissue Specificity

Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.

Database Links

HGNC: 7634

OMIM: 600355

KEGG: hsa:4671

STRING: 9606.ENSP00000428657

UniGene: Hs.646951

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Code	CSB-RA579492A0HU
Size	US$210
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Image	IHC image of CSB-RA579492A0HU diluted at 1:80 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.48% DAB.
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NAIP Recombinant Monoclonal Antibody

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