Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; BIRC5_HUMAN; EPR 1; IAP4; Survivin variant 3 alpha; SVV; TIAP
Species
Homo sapiens (Human)
Expression Region
1-142aa
Target Protein Sequence
MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human BIRC5 contains amino acids 1-142. The expected molecular weight for the BIRC5 protein is calculated to be 32.5 kDa. Expression of this BIRC5 protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of BIRC5, making it easier to detect and purify the BIRC5 recombinant protein in the later stages of expression and purification.
Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as Apoptosis inhibitor survivin, is a focus of attention in cancer research. As a protein that inhibits apoptosis, BIRC5 is overexpressed in cancer cells and closely associated with tumor development, treatment resistance, and patient prognosis. Research in the field of cancer biology has revealed that high expression of BIRC5 is correlated with the malignancy and drug resistance of various cancers, making it a hot target for cancer therapy. Inhibiting BIRC5 may help induce apoptosis in tumor cells, enhancing the effectiveness of treatment. Additionally, BIRC5 is involved in the regulation of the cell cycle, playing a role in maintaining the survival and function of normal cells. In biomedical research, there is ongoing exploration of interventions targeting BIRC5 as a means of treating other diseases, such as neurological disorders and inflammatory conditions.