Recombinant Human Branched-chain-amino-acid aminotransferase, mitochondrial (BCAT2)

Code CSB-YP002601HU
MSDS
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Source Yeast
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Code CSB-EP002601HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002601HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002601HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BCAT2
Uniprot No.
Alternative Names
BCAM; BCAT 2; BCAT(m); BCAT2; BCAT2_HUMAN; BCATM; BCT2; Branched chain aminotransferase 2; mitochondrial; Branched-chain-amino-acid aminotransferase; ECA40; mitochondrial; Placental protein 18; PP18
Species
Homo sapiens (Human)
Expression Region
28-392
Target Protein Sequence
ASS SFKAADLQLE MTQKPHKKPG PGEPLVFGKT FTDHMLMVEW NDKGWGQPRI QPFQNLTLHP ASSSLHYSLQ LFEGMKAFKG KDQQVRLFRP WLNMDRMLRS AMRLCLPSFD KLELLECIRR LIEVDKDWVP DAAGTSLYVR PVLIGNEPSL GVSQPTRALL FVILCPVGAY FPGGSVTPVS LLADPAFIRA WVGGVGNYKL GGNYGPTVLV QQEALKRGCE QVLWLYGPDH QLTEVGTMNI FVYWTHEDGV LELVTPPLNG VILPGVVRQS LLDMAQTWGE FRVVERTITM KQLLRALEEG RVREVFGSGT ACQVCPVHRI LYKDRNLHIP TMENGPELIL RFQKELKEIQ YGIRAHEWMF PV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.
Gene References into Functions
  1. identification of a peroxide-sensitive redox switch at the CXXC motif PMID: 12119021
  2. Studies of ketimine and pyridoxamine phosphate forms of BCAT@ reaction intermediates reveal substrate specificity for L-branched chain amino acids via a group of hydrophobic residues that form three hydrophobic surfaces and lock the side chain in place. PMID: 12269802
  3. role of cysteine residues in the regulatory CXXC motif PMID: 15182179
  4. ption of the CXXC center results in altered substrate orientation and deprotonation of the amino group of pyridoxamine 5'-phosphate, which inhibits catalysis PMID: 17050531
Subcellular Location
[Isoform A]: Mitochondrion.; [Isoform B]: Cytoplasm.
Protein Families
Class-IV pyridoxal-phosphate-dependent aminotransferase family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 977

OMIM: 113530

KEGG: hsa:587

STRING: 9606.ENSP00000322991

UniGene: Hs.512670

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