Recombinant Human Breast cancer metastasis-suppressor 1 (BRMS1), partial

Code CSB-RP048144h
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
BRMS1
Uniprot No.
Research Area
Apoptosis
Alternative Names
AV003220; AW554636; Breast cancer metastasis suppressor 1; Breast cancer metastasis-suppressor 1; BRMS1; BRMS1_HUMAN; DKFZp564A063 ; MGC95128
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-235aa
Target Protein Sequence
MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
54.3kDa
Protein Length
Partial
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Gene References into Functions
  1. BRMS1FANCI interaction is necessary for the regulatory role of BRMS1 in the FA pathway. PMID: 30365131
  2. overexpression of miR-3200-5p significantly decreased BRMS1 levels and promoted OS cell invasion and migration, while depletion of miR-3200-5p significantly increased BRMS1 levels and inhibited OS cell invasion and migration. Study revealed that miR-3200-5p may be a critical regulator of OS cell invasiveness. PMID: 29890825
  3. Low BRMS1 expression is associated with Hepatocellular carcinoma. PMID: 29295726
  4. Low BRMS1 expression is associated with high metastatic capacity of breast cancer. PMID: 29970691
  5. High BRMS1 Expression is associated with Metastases and recurrence in Lung Adenocarcinoma. PMID: 29097253
  6. The results showed that reduction in the breast cancer metastasis suppressor 1 [BRMS1] expression level was linked directly to clinico-pathological features of breast cancer significantly. The loss of expression or reduced levels of BRMS1 is potentially a strong indicator of the metastatic capacity of breast cancer with poor prognosis. PMID: 28533425
  7. The RAS-related nuclear protein ((P) ran), breast cancer metastasis suppressor 1 ((P) brms1) and minichromosome maintenance complex component 5 ((P) mcm5) promoters have the specificity and strength needed for cancer-specific expression-targeted gene therapy. PMID: 27140445
  8. we identify a therapeutically exploitable posttranslational mechanism by which CK2alpha-mediated degradation of BRMS1 promotes metastases in lung cancer PMID: 26980766
  9. our study characterized DAPK1 as a novel transcriptional target of BRMS1. Transcriptional activation of DAPK1 might be another important mechanism accounting for the metastasis suppressive activity of BRMS1. PMID: 28339067
  10. BRMS1 promoter methylation and aberrant protein expression seem to be related to high-risk types of human papilloma virus-induced carcinogenesis in uterine cervix. PMID: 28381193
  11. miR-346 promotes migration and invasion of nasopharyngeal carcinoma cells via targeting BRMS1. PMID: 27501413
  12. A novel link has been discussed between CDK2 expression and cell migration by characterizing the CDK2-mediated phosphorylation of BRMS1. PMID: 26730572
  13. Phosphorylation of BRMS1 by CDK2 regulates the migration of tumor cells. PMID: 26771717
  14. Data show that Cullin3 exerts its function through promoting breast-cancer metastasis suppressor 1 (BRMS1) protein degradation, which was associated with epithelial-mesenchymal transition (EMT), migration and invasion. PMID: 26544623
  15. The studies reviewed here with respect to BRMS1 structure, cellular effects, intracellular signaling, and clinical value consolidate the importance of BRMS1 in the development of metastasis. PMID: 26328523
  16. Aberrant methylation of BRMS1 frequently occurs in the down-regulation of BRMS1 in triple negative breast cancer and that it may play a role in the metastasis of breast cancer. PMID: 26617826
  17. the present study demonstrates a mechanical cascade of BRMS1 suppressing cancer cell invasion through downregulating HIF-1alpha transcript and consequently reducing Snail and TWIST1 expression. PMID: 26520789
  18. MRTF-A and STAT3 synergistically recruited DNMT1 to hypermethylate the promoter of BRMS1 and affect the expression of BRMS1.MRTF-A and STAT3 promote breast cancer cell migration via hypermethylating BRSM1. PMID: 25854163
  19. BRMS1 expression in human breast cancer is negatively correlated with JARID1C expression. Our results, for the first time, portray a pivotal role of JARID1C in regulating metastatic behaviors of breast cancer cells PMID: 26182878
  20. high expression of BRSM1 in rectal cancer plays an essential role in tumor progression PMID: 24748145
  21. loss of BRMS1 promotes malignant phenotypes that are dependent on NF-kappaB-dependent regulation of Twist1 PMID: 25368381
  22. BRMS1 is a key regulator required to maintain a cellular morphology and cytoskeletal architecture consistent with an epithelial phenotype. PMID: 24763730
  23. BRMS1 overexpression inhibited glioma cell invasion. PMID: 24879377
  24. BRMS1-expressing cells remained rounded. PMID: 24000122
  25. Silencing of BRMS 1 significantly induced the expression of NF-kappaB subunit, p65, uPA, and OPN proteins PMID: 24984534
  26. Methylation of BRMS1 promoter in cfDNA isolated from plasma of NSCLC patients provides important prognostic information and merits to be further evaluated as a circulating tumour biomarker. PMID: 24642624
  27. Data define BRMS1 promoter methylation in primary breast tumors and associated circulating tumor cells. PMID: 23744981
  28. Low levels of BRMS1 were observed in patients with high-grade tumors, in patients with distant metastasis in breast cancer. PMID: 24596389
  29. BRMS1 SNP rs1052566 heterozygous individuals were more likely to have node-positive breast tumors. PMID: 23771732
  30. Report BRMS1 transcript variant which regulates heptocellular carcinoma apoptosis and growth. PMID: 23643861
  31. Authors observed that residues 85 to 98 might be important in defining the oligomerization state of the BRMS1 N-terminal coiled coil. PMID: 23500495
  32. The C-terminal putative nuclear localization sequence (NSL2) of BRMS1 is necessary for metastasis suppression. PMID: 23390556
  33. Data suggest that low expression of the metastasis suppressor BRMS1 may be an independent prognostic factor for poor prognosis in nasopharyngeal carcinoma (NPC) patients. PMID: 22931099
  34. The expression of BRMS1 protein in supraglottic cancer is significantly decreased. BRMS1 gene promotor methylation is related with down-expression of BRMS1 protein. PMID: 23167184
  35. Data indicate that mutation of E3 ligase motif not only abolishes BRMS1-induced p300 polyubiquitination and degradation, but importantly, dramatically reduces the metastasis suppressor function of BRMS1. PMID: 23269275
  36. BRMS1 sensitizes HCC cells to apoptosis through suppressing OPN expression PMID: 22927944
  37. The loss of BRMS1 expression may be involved in the development and progression of nasal and paranasal sinus carcinomas. PMID: 22239051
  38. Loss of breast cancer metastasis suppressor 1 promotes ovarian cancer cell metastasis by increasing chemokine receptor 4 expression. PMID: 22200669
  39. high level of expression and lack of promoter methylation are associated with better overall survival in non-small cell lung cancer patients PMID: 21726917
  40. These results suggest that the novel regulatory mechanism of BRMS1 by Cul3-SPOP complex is important for breast cancer progression. PMID: 22085717
  41. SATB1 and BRMS1 might play an important role in the development and lymph node metastasis of ovarian cancer. PMID: 21355308
  42. possible link between BRMS1 expression and apoptosis in human breast cancer through a relationship with the expression of genes belonging to the X-chromosome RBM family. PMID: 21737612
  43. the enigmatic complexities of BRMS1-mediated metastasis suppression PMID: 21827753
  44. The expression of BRMS1 protein in supraglottic cancer is significantly decreased. Expression has a close relationship with pathologic differentiation and clinical stage and cervical lymph node metastasis. PMID: 19621595
  45. Expression of BRMS1 mRNA in supraglottic cancer is lower than that in adjacent normal mucosa. PMID: 18533556
  46. BRMS1 expression was decreased in metastatic melanomas, which resulted in deficient suppression of angiogenesis and contributed to melanoma progression. PMID: 20935672
  47. Study observed that SNX6 increases BRMS1-dependent transcriptional repression. Moreover, over-expression of SNX6 was capable of diminishing trans-activation in a dose-dependent manner. PMID: 20830743
  48. Patients with high levels of expression of BRMS1 mRNA have a better prognosis than those with low expression. PMID: 17085653
  49. ING4 is induced by BRMS1 and that it inhibits melanoma angiogenesis by suppressing NF-kappaB activity and IL-6 expression. PMID: 21056991
  50. BRMS1 expression in breast cancer cells induced reorganization of F-actin and caused alteration in cytoarchitectures (cell topography and ultrastructure). PMID: 20083343

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Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly nuclear.
Protein Families
BRMS1 family
Tissue Specificity
Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
Database Links

HGNC: 17262

OMIM: 606259

KEGG: hsa:25855

STRING: 9606.ENSP00000396052

UniGene: Hs.100426

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