Recombinant Human C-Myc-binding protein (MYCBP)

Code CSB-EP858710HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MYCBP
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
AMY 1; AMY-1; AMY1; Associate of Myc 1; C myc binding protein ; C-Myc-binding protein; Cmyc binding protein; MYCBP; MYCBP_HUMAN
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-103aa
Target Protein Sequence
AHYKAADSKREQFRRYLEKSGVLDTLTKVLVALYEEPEKPNSALDFLKHHLGAATPENPEIELLRLELAEMKEKYEAIVEENKKLKAKLAQYEPPQEEKRAE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
27.8kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
May control the transcriptional activity of MYC. Stimulates the activation of E box-dependent transcription by MYC.
Gene References into Functions
  1. c-Myc expression is reduced in pterygium compared with normal conjunctiva. PMID: 22050566
  2. Suppression of telomerase activity mediated by PinX1 is involved in the Mad1/c-Myc pathway. PMID: 20544396
  3. miR-22 acts as a tumor suppressor through direct repression of MYCBP expression and subsequent reduction of oncogenic c-Myc activities. PMID: 20562918
  4. identified a novel AMY-1-binding protein, AAT-1, and determied its expression profiles, genes, and cellular localization; results showed that AAT-1 was specifically expressed in the testis throughout spermatogenesis and was also present in mature sperm PMID: 12223483
  5. interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits enzyme acdtivity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex PMID: 12414807
  6. AMY-1 coordinates ADP-Ribosylation Factors-mediated membrane trafficking and signaling pathways. PMID: 16866877
  7. An important role for MBP-1 in the altered cell proliferation and energy utilization that occur in response to an altered glucose concentration. PMID: 17595061
  8. AMY-1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggest that AMY-1 plays a role in spermatogenesis PMID: 11483602

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Subcellular Location
Cytoplasm. Nucleus. Mitochondrion. Note=Translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression. Found in the mitochondria when associated with AKAP1.
Protein Families
AMY1 family
Tissue Specificity
Highly expressed in heart, placenta, pancreas, skeletal muscle and kidney. Also present at low levels in lung.
Database Links

HGNC: 7554

OMIM: 606535

KEGG: hsa:26292

STRING: 9606.ENSP00000380702

UniGene: Hs.591506

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