Recombinant Human C-type lectin domain family 6 member A (CLEC6A), partial

Code CSB-YP738603HU
MSDS
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Source Yeast
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Code CSB-EP738603HU
MSDS
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Source E.coli
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Code CSB-EP738603HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP738603HU
MSDS
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Source Baculovirus
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Code CSB-MP738603HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLEC6A
Uniprot No.
Alternative Names
C type lectin domain family 6 member A; C type lectin superfamily member 10; C-type (calcium dependent; carbohydrate-recognition domain) lectin; superfamily member 10; C-type lectin domain family 6 member A; C-type lectin superfamily member 10; CLC6A_HUMAN; CLEC4N; CLEC4N; mouse; homolog of; Clec6a; CLECSF10; DC associated C type lectin 2; DC-associated C-type lectin 2; Dectin-2; Dectin2; Dendritic cell associated lectin 2; Dendritic cell-associated C-type lectin 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: specifically recognizes and binds alpha-mannans on C.albicans hypheas. Binding of C.albicans alpha-mannans to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses.
Gene References into Functions
  1. functions in Kupffer cells to mediate the uptake and clearance of cancer cells PMID: 27872290
  2. Data suggest that the specificity and geometry of dectin-2/CLEC6A carbohydrate-binding site provide molecular mechanism for binding of dectin-2 to fungal mannans and also to bacterial lipopolysaccharides, capsular polysaccharides, and lipoarabinomannans that contain mannose-alpha-1--2-mannose disaccharide unit. (CLEC6A = C-type lectin domain containing 6A) PMID: 28652405
  3. these data indicate that mannosylated O-antigens from several Gram-negative bacteria augment TLR4 responses through interaction with Dectin-2. PMID: 27358401
  4. This study showed a genetic association between Dectin-2 and pulmonary cryptococcosis. PMID: 26129889
  5. human plasmacytoid dendritic cells directly recognize A. fumigatus hyphae via Dectin-2 PMID: 25659141
  6. our data show a requirement for Dectin-2 in alveolar macrophage induction of cysteinyl leukotrienes and in the initiation of airway inflammation. PMID: 24129160
  7. The Dectin-2 possess a single extracellular conserved C-type lectin-like domain and are capable of mediating intracellular signalling either directly, through integral signalling domains, or indirectly, by associating with signalling adaptor molecules. PMID: 23606632
  8. human DECTIN-2 has a role in innate immunity PMID: 15810886

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Tissue Specificity
Expressed in lung, spleen, lymph node, leukocytes, bone marrow, tonsils and dendritic cells. Strongly expressed in purified monocytes and weakly in B-cells. In peripheral blood cells, preferentially expressed in plasmacytoids rather than myeloids.
Database Links

HGNC: 14556

OMIM: 613579

KEGG: hsa:93978

STRING: 9606.ENSP00000371505

UniGene: Hs.591147

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