Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Cambridge pathology 1 antigen; CAMPATH 1; CAMPATH 1 antigen; CAMPATH-1 antigen; CD 52; CD52; CD52 antigen; CD52 molecule; CD52_HUMAN; CDw52; CDW52 antigen; Epididymal secretory protein E5; Epididymis secretory sperm binding protein Li 171mP; HE 5; He5; Human epididymis-specific protein 5
Species
Homo sapiens (Human)
Expression Region
25-36aa
Target Protein Sequence
GQNDTSQTSSPS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant human CD52 protein is encoded by a recombinant DNA that was cloned into the expression vector and then transformed into the E.coli that supports the expression of the gene. The recombinant DNA was constructed by fusing the N-terminal 6xHis tag gene to the gene fragment coding for the 25-36aa of the human CD52 protein. After purification, the product is the recombinant human CD52 protein. This recombinant CD52 protein was subjected to the SDS-PAGE determination. Its purity reaches over 90% evaluated by Bandscan software analysis combined with SAS-PAGE. This CD52 protein ran to the molecular weight of about 20 kDa under SDS-PAGE condition.
CD52 is called a glycoprotein, which often is expressed in the surface of mature lymphocytes, monocyte or dendritic cells. CD52 protein consists of 12 amino acids, which anchored to glycosylphosphatidylinositol (GPI). It also acts to restrain the cells adhesion, which contributes to the freely move of cells. Studies implied that CD52 is related to some types of lymphoma. CD52 Is elevated on B cells of systemic lupus erythematosus (SLE) patients and regulates B cell function. The CD52 targeted agent alemtuzumab has been on the market to improve the treatment of chronic lymphocytic leukemia.