Human CAMPATH-1 antigen(CD52) ELISA kit

Instructions
Code CSB-EL004943HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CD52 molecule
Alternative Names Cambridge pathology 1 antigen ELISA Kit; CAMPATH 1 ELISA Kit; CAMPATH 1 antigen ELISA Kit; CAMPATH-1 antigen ELISA Kit; CD 52 ELISA Kit; CD52 ELISA Kit; CD52 antigen ELISA Kit; CD52 molecule ELISA Kit; CD52_HUMAN ELISA Kit; CDw52 ELISA Kit; CDW52 antigen ELISA Kit; Epididymal secretory protein E5 ELISA Kit; Epididymis secretory sperm binding protein Li 171mP ELISA Kit; HE 5 ELISA Kit; He5 ELISA Kit; Human epididymis-specific protein 5 ELISA Kit
Abbreviation CD52
Uniprot No. P31358
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 ng/mL-5000 ng/mL
Sensitivity 19.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD52 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %98
Range %91-105
1:4Average %96
Range %92-100
1:8Average %96
Range %89-102
Recovery
The recovery of human CD52 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9080-100
EDTA plasma (n=4)9891-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
50001.975 1.952 1.964 1.870
25001.612 1.586 1.599 1.505
12501.272 1.171 1.222 1.128
6250.984 0.932 0.958 0.864
3120.665 0.629 0.647 0.553
1560.398 0.368 0.383 0.289
780.193 0.201 0.197 0.103
00.097 0.091 0.094  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Function
(From Uniprot)
May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Gene References into Functions
  1. soluble CD52 exerts a concerted immunosuppressive effect by first sequestering HMGB1 to nullify its proinflammatory Box B, followed by binding to the inhibitory Siglec-10 receptor, triggering recruitment of SHP1 to the intracellular immunoreceptor tyrosine-based inhibitory motif of Siglec-10 and its interaction with the TCR. PMID: 29997173
  2. CD52 is a novel prognostic NSC marker and a potential NSC target in a subset of patients with MDS and AML, which may have clinical implications and may explain clinical effects produced by alemtuzumab in these patients. PMID: 24799522
  3. carbohydrate moiety of sperm protein interferes with the complement system via binding to C1q PMID: 22386526
  4. CT60 single-nucleotide polymorphism of CTLA4 is a surrogate marker for donor lymphocyte infusion outcome after allogeneic cell transplantation for acute leukemia PMID: 21552305
  5. Clonal large granular lymphocytes exhibited decreased CD52 expression post-therapy in patients refractory to treatment. PMID: 19794084
  6. Our bioinformatics findings suggest that CD52 polymorphism may affect the efficiency of GPI anchor formation and thus may indirectly alter the response to anti-CD52 agents like alemtuzumabin renal transplantation. PMID: 20349607
  7. Review article on CD52 structure and function. PMID: 11860230
  8. HE5(CD52) mRNA and protein, expressed in epithelial cells of the distal epididymis, were not affected by the obstruction of the vas deferens. PMID: 14662784
  9. The relationship between this differential insertion and differences in glycosylation of rat and human CD52 is discussed. PMID: 16266689
  10. CD52 is widely expressed on human mast cells (MCs) and Waldenstrom's Macroglobulinemia bone marrow lymphoplasmacytic cells and provide the preclinical rationale for the use of alemtuzumab in the treatment of WM and possibly other MC-related disorders. PMID: 16796779
  11. In this study, we identified the antigen of 4C8 mAb as CD52. CD52 is a costimulatory molecule for induction of CD4-positive T cells. PMID: 16797237
  12. In contrast with CLL, the variable expression of CD52 among other hematologic malignancies suggests that target validation on a case-by-case basis will likely be necessary to guide the rational analysis of CAMPATH therapy. PMID: 17145843
  13. Our data demonstrate differences in the intensity of the CD52 antigen expression between B-lymphocytes and tumor lymphocytes of B-CLL patients, and between B-CLL and SLL tumor cells. CD52 antigen is expressed at low level on CD34(+) cells. PMID: 17428002
  14. The semenogelin-CD52 soluble form is a direct consequence of the liquefaction process in human semen. PMID: 17624925
  15. We first showed the expression of CD52 in human cumulus cells. CD52 has some functional roles around fertilization in females as well as in males. PMID: 18647288
  16. A review on CD52 expression and function PMID: 11257744

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Database Links

HGNC: 1804

OMIM: 114280

KEGG: hsa:1043

STRING: 9606.ENSP00000363330

UniGene: Hs.276770

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