Human CAMPATH-1 antigen(CD52) ELISA kit

Code CSB-EL004943HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P31358
Lead Time 7-14 working days
Abbreviation CD52
Target Name CD52 molecule
Alias CDW52, CD52 antigen|CD52 antigen (CAMPATH-1 antigen)|CDW52 antigen (CAMPATH-1 antigen)
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 ng/mL-5000 ng/mL
Sensitivity 19.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 1804
MGI 1346088
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD52 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %98
Range %91-105
1:4Average %96
Range %92-100
1:8Average %96
Range %89-102
Recovery
The recovery of human CD52 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9080-100
EDTA plasma (n=4)9891-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
50001.975 1.952 1.964 1.870
25001.612 1.586 1.599 1.505
12501.272 1.171 1.222 1.128
6250.984 0.932 0.958 0.864
3120.665 0.629 0.647 0.553
1560.398 0.368 0.383 0.289
780.193 0.201 0.197 0.103
00.097 0.091 0.094  
References
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Function May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Database Links

HGNC: 1804

OMIM: 114280

KEGG: hsa:1043

STRING: 9606.ENSP00000363330

UniGene: Hs.276770

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