Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

CNN2

Uniprot No.

Q99439

Research Area

Signal Transduction

Alternative Names

Calponin 2; Calponin H2; Calponin H2, smooth muscle; Calponin-2; Cnn2; CNN2_HUMAN; Neutral calponin; smooth muscle

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-309aa

Target Protein Sequence

SSTQFNKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIGPDFQKGLKDGTILCTLMNKLQPGSVPKINRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDLFESGNMTQVQVSLLALAGKAKTKGLQSGVDIGVKYSEKQERNFDDATMKAGQCVIGLQMGTNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQMGTNKCASQVGMTAPGTRRHIYDTKLGTDKCDNSSMSLQMGYTQGANQSGQVFGLGRQIYDPKYCPQGTVADGAPSGTGDCPDPGEVPEYPPYYQEEAGY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

49.6kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human CNN2 was expressed with the amino acid range of 2-309. This CNN2 protein is expected to have a theoretical molecular weight of 49.6 kDa. This CNN2 recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of CNN2, making it easier to detect and purify the CNN2 recombinant protein in the later stages of expression and purification.

Human Calponin-2 (CNN2) is a cytoskeletal protein belonging to the calponin family, involved in the regulation of smooth muscle contraction and actin cytoskeleton organization. Encoded by the CNN2 gene, it is primarily expressed in smooth muscle tissues, such as those in the uterus, intestine, and vascular walls. CNN2 interacts with actin and calmodulin, influencing actin-myosin dynamics. Its role in smooth muscle contraction suggests implications in physiological processes like vascular tone and uterine contractions. Additionally, CNN2 has been associated with various diseases, including cancer, where its altered expression may contribute to tumor progression. The study of human CNN2 contributes to understanding smooth muscle function, cytoskeletal regulation, and its potential relevance in disease mechanisms.

Target Background

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Gene References into Functions

Study in human hepatocellular carcinoma cell line and xenograft mouse model shows that CNN2 plays an important role in tumor growth and metastasis, possibly through MEK1/2-ERK1/2 signaling pathway. PMID: 29333089
our results demonstrated that knockdown of endogenous CNN2 in AGS cells could significantly activate cell apoptosis pathway and therefore suppress cell growth in vitro. The deletion of CNN2 might be a potential therapeutic approach to inhibit aggressive growth of gastric cancer. PMID: 28714360
High Calponin-h2 serum levels are associated with breast cancer. PMID: 25099617
h2-Calponin gene expression is further affected by the treatment of cells with Notch inhibitor and activator, suggesting an upstream signaling mechanism. PMID: 24285540

Protein Families

Calponin family

Tissue Specificity

Heart and smooth muscle.

Database Links

HGNC: 2156

OMIM: 602373

KEGG: hsa:1265

STRING: 9606.ENSP00000263097

UniGene: Hs.651512

Code	CSB-EP860764HU
Abbreviation	Recombinant Human CNN2 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Calponin-2 (CNN2)

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